Analysis of the transport of and cytotoxic effects for nalbuphine solution in corneal cells.

Objective: To assess the in vitro effects of various nalbuphine concentrations on viability and wound healing ability of corneal cells and potential drug transport through the corneal epithelium.

Sample: Cultured canine and human corneal epithelial cells (CECs) and cultured canine corneal stromal fibroblasts.

Procedures: CECs and stromal fibroblasts were exposed to nalbuphine (concentration of solutions ranged from 0% to 1.