Polymerase chain reaction-based identification of various serotypes of in adults with and without dental caries.

Background: Dental caries is a multistep process which initiates the development of plaque' defined as a structured biofilm containing microbial communities. Teeth provide unique surfaces for bacterial colonization. Serotypes of implicate the development of dental caries.

Development of Automated Risk Stratification for Sporadic Odontogenic Keratocyst Whole Slide Images with an Attention-Based Image Sequence Analyzer.

(1) Background: The categorization of recurrent and non-recurrent odontogenic keratocyst is complex and challenging for both clinicians and pathologists. What sets this cyst apart is its aggressive nature and high likelihood of recurrence. Despite identifying various predictive clinical/radiological/histopathological parameters, clinicians still face difficulties in therapeutic management due to its inherent aggressive nature.

Building Automation Pipeline for Diagnostic Classification of Sporadic Odontogenic Keratocysts and Non-Keratocysts Using Whole-Slide Images.

The microscopic diagnostic differentiation of odontogenic cysts from other cysts is intricate and may cause perplexity for both clinicians and pathologists. Of particular interest is the odontogenic keratocyst (OKC), a developmental cyst with unique histopathological and clinical characteristics. Nevertheless, what distinguishes this cyst is its aggressive nature and high tendency for recurrence.

Comparison of culture and polymerase chain reaction-restriction fragment length polymorphism for identification of various species from subgingival plaque samples of healthy and periodontally diseased individuals.

Introduction: are facultative anaerobic Gram-negative bacilli and recognized as opportunistic pathogens of various extraoral infections. Only a few studies attempted to identify all the seven species of phenotypically and genotypically in healthy individuals and patients with chronic periodontitis. Studies to determine the prevalence of in subgingival plaque samples from healthy individuals, chronic gingivitis and periodontitis among Indian population are lacking.

Detection and comparison of prevalence of through culture and Real Time-polymerase chain reaction in subgingival plaque samples of chronic periodontitis and healthy individuals.

Introduction: The micro-flora of oral cavity is a myriad of micro-organism. Any infection of oral cavity leads to diseased condition which is a transitional transformation of the micro-organism in a specific paradigm depending upon the diseased condition. Periodontitis is one of the predominant chronic diseases which is a multifactorial infection.

Phenotypic Expression of Oral Fibroblasts Derived from Oral Submucous Fibrosis: An Assay through Cell Culture.

Objective: The present study aimed to establish cell lines of fibroblast from human OSF tissues and their response to varying concentrations of arecoline. The various morphological forms of fibroblasts were identified to establish phenotypic change.

Materials And Method: Fibroblast cell lines were obtained from control samples as well as from OSF cases.

Idiopathic first bite syndrome - A rare case report with review of literature.

First bite syndrome (FBS) is a condition that classically presents as severe pain in the preauricular region, initiated on the first bite of a meal. In most of the cases reported, it is associated with a history of upper neck surgery or tumor of the parotid salivary gland or parapharyngeal space (PPS). Some propose that FBS arises due to damage to the cervical sympathetic trunk leading to the loss of sympathetic innervations to the parotid salivary gland.

Molecular identification of species from the oral cavity of patients with chronic periodontitis and healthy individuals.

Introduction: species are recognized as part of human oral microbiota and implicated as periodontal pathogens associated with various periodontal diseases. The three original species - and were initially isolated from periodontitis in adults, but subsequent studies demonstrated their presence also at periodontally healthy sites in both children and adults. Their association with periodontal disease is a matter of controversy.

Comparison of cluster analysis of by arbitrarily primed-polymerase chain reaction between healthy and chronic periodontitis subjects.

Introduction: Periodontitis is a chronic destructive inflammatory disease of the oral cavity. The main causative agent is presence of biofilm formed due to different micro-organisms. Among different micro- organisms "red complex" bacteria is known to be the main causative agent in progression of periodontitis.

Comparative Evaluation of Immunohistochemical Expression of p16 with p16 Microsatellite Marker by PCR in Surgical Margins of Oral Squamous Cell Carcinoma.

Oral squamous cell carcinoma (OSCC) is most common oral cancer with multifactorial etiology. Surgical therapy is treatment of choice but known to have recurrence. The main reason for recurrence is associated with surgical margins which need to be tumor free.

Cell-free tumor DNA: Emerging reality in oral squamous cell carcinoma.

The intention of this review was to condense ongoing findings on the use of circulating DNAs from bodily fluids (blood, serum and plasma) as cancer biomarkers in patients with oral squamous cell carcinoma. Studies were collected after searching databases: PubMed and Google library. Additional search was performed through cross-check on the bibliography of selected articles.

culture of oral keratinocytes using direct explant cell culture technique.

Background: Culture of cells and tissues is a standard research method practiced in many laboratories. In most of the cases, these cultures are being used as substrates for cell products or as investigative tools for delving the mechanism of gene expression, cell proliferation and transformation. Primary monolayer cell culture has been beneficial to study the general biology of both oral and skin keratinocytes.

Detection and comparison of microsatellite marker D9S1747 with clinical stages and grades of oral squamous cell carcinoma.

Background: One of the main characteristics of oral squamous cell carcinoma (OSCC) is genetic alteration in specific target regions. Allelic imbalance in tumor suppressor genes is the key event in OSCC which is associated with loss of heterozygosity mostly on chromosome 9p21 locus which includes p16 marker. p16 (D9S1747) is a microsatellite marker which detects early changes in OSCC.

Forensic application of frontal sinus measurement among the Indian population.

Aim: Studies have been done in diversified population, demonstrating the uniqueness of frontal sinus; data related to the Indian population are less. Thus, the present study was aimed to determine the frontal sinus measurement and to assess its forensic application in the Indian population.

Materials And Methods: A total of 400 individuals with Indian origin (21-30 years) were included in the study.

Detection of antibodies against Aggregatibacter actinomycetemcomitans in serum and saliva through ELISA in periodontally healthy individuals and individuals with chronic periodontitis.

Background: Periodontitis is a persistent polymicrobial infection, which leads to chronic inflammation in the tooth supporting tissues. Aggregatibacter actinomycetemcomitans are normal commensals of oral cavity but are low in number in periodontally healthy subjects. They are one of the major pathogens aetiologically linked to periodontal disease.

Comparison of microvessel density using CD34 and CD105 in oral submucous fibrosis and its correlation with clinicopathological features: An immunohistochemical study.

Objectives: To compare immunohistochemical expression of CD34 and CD105 in oral submucous fibrosis (OSF) and normal oral mucosa (NOM) and correlate with clinicopathological features of OSF.

Materials And Methods: Total of thirty clinically diagnosed and histologically confirmed cases of OSF and 15 NOM were included in the study. Tissues sections were immunostained using CD34 and CD105 antibodies.

Comparison of palatal rugae pattern among Indian and Tibetan population.

Introduction: Palatoscopy is the study of palatal rugae pattern to establish the identity of a person. The palatal rugae are permanent and unique to each person and can establish identity through discrimination (via casts, tracings, or digitized rugae patterns). In addition, rugae pattern may be specific to racial groups facilitating population identification (which may require postdisasters).

Comparison of immunohistochemical expression of vascular endothelial growth factor and CD105 in oral squamous cell carcinoma: Its correlation with prognosis.

Objectives: Compare and correlate immunoexpression of vascular endothelial growth factor (VEGF) and CD105 in oral squamous cell carcinoma (OSCC), and correlate its expression with the prognosis of the patient.

Materials And Methods: A retrospective study was carried out on total of 49 cases of OSCC. Detailed demographic and clinical data were obtained, and tissue sections were stained with hematoxylin and eosin to grade the tumor.

Multiplex polymerase chain reaction detection of selected bacterial species from symptomatic and asymptomatic non-vital teeth with primary endodontic infections.

Aim: The aim of the present study was to investigate the presence of selective anaerobic microorganisms in primary root canal infections of symptomatic and asymptomatic non-vital teeth with periapical pathosis using multiplex polymerase chain reaction.

Methods: A total of 100 root canal samples (50 from symptomatic and 50 from asymptomatic teeth) were obtained from patients with primary endodontic infections. DNA extracted from the samples was amplified by using specific primers for the 16S rRNA gene of each bacterium, and semiquantification was done to analyze the prevalence of microorganisms and their correlation to clinical features.

Culture-based identification of pigmented Porphyromonas and Prevotella species in primary endodontic infections.

Background. The most common species isolated from primary endodontic infections are black-pigmented bacteria. These species are implicated in apical abscess formation due to their proteolytic activity and are fastidious in nature.

Do the presence of Barr bodies in male jail inmates indicates criminality: A pilot study.

Background: Cytogenetic studies from past decades have shown that interphase cells of female cats contain a densely stained chromatin mass in their nuclei called as Barr bodies (BBs) named after the scientist Murray Barr. BBs are unique chromatin structures formed due to the condensation of the X-chromosome. Many psychopathic disorders originate from defective genes including the multiple X syndromes.

Immunohistochemical Expression of Podoplanin in Clinical Variants of Oral Leukoplakia and Its Correlation With Epithelial Dysplasia.

Oral leukoplakia is the most common premalignant lesion of the oral cavity and is associated with development of oral squamous cell carcinoma. Certain changes at cellular and molecular level are important indicators for transformation into carcinoma. Podoplanin, a transmembrane glycoprotein is involved in the cytoskeletal remodeling and increased motility of the cell which helps in determining the malignant potential of oral leukoplakic lesions.

Radiographic assessment of facial soft tissue thickness in South Indian population--An anthropologic study.

Facial reconstruction is a technique used in forensic anthropology to identify an unknown person. Various methods used for facial reconstruction are drawings, sculpture and computer aided image building which is mainly based on facial soft tissue thickness measurement. Several methods have been established for measuring facial soft tissue thickness (FSTT) with each one having certain limitations.

Immunohistochemical Expression of CD105 and TGF-β1 in Oral Squamous Cell Carcinoma and Adjacent Apparently Normal Oral Mucosa and its Correlation With Clinicopathologic Features.

Angiogenesis in oral squamous cell carcinomas (OSCC) is essential for its growth, invasion, and metastasis. This entails a shift in the balance between proangiogenic and antiangiogenic factors. CD105 and TGF-β1 are 2 such proangiogenic factors wherein CD105 exerts its angiogenic effect by binding to and modulating the TGF-β1 pathway.

Histomorphometric study to compare histological changes between oral squamous cell carcinoma and apparently normal adjacent oral mucosa.

Despite the advances in surgery, radiotherapy and chemotherapy the annual death for oral squamous cell carcinoma (OSCC) is rising rapidly. The carcinoma has propensity to develop in a field of cancerization. Clinically may it be apparently normal mucosa (ANM) adjacent to squamous cell carcinoma which harbours certain discrete molecular alteration which ultimately reflects in cellular morphology.