Publications by authors named "Vijaya Rachel Kappala"
Triple negative breast cancer is a clinically challenging subtype due to lack of biomarker for rational targeted therapy. Lipid rafts are cholesterol enriched rigid platforms, which colocalize signalling molecules of cancer progression. This study explores the effect of lipid rafts disruption by cholesterol depleting agent, MβCD on induction of apoptosis and expression of WNT receptor LRP6, survivin and common apoptotic markers in TNBC cell lines.
View Article and Find Full Text PDFArticle Synopsis
- Lipid rafts, which are cholesterol-rich microdomains, play a role in cell signaling related to cancer progression, and this study explored how cholesterol removal affects triple-negative breast cancer (TNBC) cells.
- Researchers used methyl-β-cyclodextrin (MβCD) to extract cholesterol, leading to significant cytotoxicity and reduced adhesion and invasion abilities in TNBC cells, specifically MDA-MB 231 and MDA-MB 468.
- MβCD treatment also resulted in cell cycle arrest and increased apoptosis in TNBC cells, alongside decreased neovessel formation and depletion of certain signaling receptors involved in angiogenesis.
Background: The defensive capacities of plant protease Inhibitors (PI) rely on inhibition of proteases in insect guts or those secreted by microorganisms; and also prevent uncontrolled proteolysis and offer protection against proteolytic enzymes of pathogens.
Methods: An array of chromatographic techniques were employed for purification, homogeneity was assessed by electrophoresis. Specificity, Ki value, nature of inhibition, complex formation was carried out by standard protocols.