Effect of short-term exposure to high-altitude hypoxic climate on feed-intake, blood glucose level and physiological responses of native and non-native goat.

The exposure to high altitude and cold stress poses challenges in maintaining normal physiological standards and body homeostasis in non-native animals. To enhance our understanding of the physiology of native and non-native goats in high-altitude environments, we conducted a comparative study to examine the impact of natural hypoxic and cold stress conditions on their feed intake (FIT) and associated changes in physiological responses, including plasma glucose concentration (PGC). The study took place at an altitude of 3505.

High-altitude and low-altitude adapted chicken gut-microbes have different functional diversity.

Recently, there has been considerable interest in the functions of gut microbiota in broiler chickens in relation to their use as feed additives. However, the gut-microbiota of chickens reared at different altitudes are not well documented for their potential role in adapting to prevailing conditions and functional changes. In this context, the present study investigates the functional diversity of gut-microbes in high-altitude (HACh) and low-altitude adapted chickens (LACh), assessing their substrate utilization profile through Biolog Ecoplates technology.

Recent advancements in toxicology, modern technology for detection, and remedial measures for arsenic exposure: review.

Arsenic toxicity has become a major global health concern for humans and animals due to extensive environmental and occupational exposure to arsenic-contaminated water, air, soil, and plant and animal origin food. It has a wide range of detrimental effects on animals, humans, and the environment. As a result, various experimental and clinical studies were undertaken and are undergoing to understand its source of exposures, pathogenesis, identify key biomarkers, the medical and economic impact on affected populations and ecosystems, and their timely detection and control measures.

Selection of species specific panel of reference genes in peripheral blood mononuclear cells of native livestock species adapted to trans-Himalayan region of Leh-Ladakh.

The identification of appropriate references genes is an integral component of any gene expression-based study for getting accuracy and reliability in data interpretation. In this study, we evaluated the expression stability of 10 candidate reference genes (GAPDH, RPL4, EEF1A1, RPS9, HPRT1, UXT, RPS23, B2M, RPS15, ACTB) in peripheral blood mononuclear cells of livestock species that are adapted to high altitude hypoxia conditions of Leh-Ladakh. A total of 37 PBMCs samples from six native livestock species of Leh-Ladakh region such as Ladakhi cattle, Ladakhi yak, Ladakhi donkey, Chanthangi goat, Double hump cattle and Zanskar ponies were included in this study.

Identification of Internal Reference Genes in Peripheral Blood Mononuclear Cells of Cattle Populations Adapted to Hot Arid Normoxia and Cold Arid Hypoxia Environments.

To estimate gene expression in a reliable manner, quantitative real-time polymerase chain reaction data require normalisation using a panel of stably expressed reference genes (RGs). To date, information on an appropriate panel of RGs in cattle populations reared at cold arid high-altitude hypoxia and hot arid tropical normoxia environments is not available. Therefore, the present study was carried out to identify a panel of stably expressed RGs from 10 candidate genes ( and ) in peripheral blood mononuclear cells (PBMCs) of cattle populations reared at cold arid high-altitude hypoxia and hot arid normoxia environments.

Health Risk Assessment of Heavy Metals Due to Wheat, Cabbage, and Spinach Consumption at Cold-Arid High Altitude Region.

Soil and water from the trans-Himalayan high-altitude region contain high concentrations of various heavy metals. Vegetables and cereals such as cabbage, spinach, and wheat are most prone to heavy metal accumulation from soil and water which can be toxic for human consumption. It has yet to be studied how consumption of vegetables and cereal with excess heavy metal content can affect human health in high altitude areas.

Health Risk Assessment of Heavy Metals Through Cow Milk Consumption in Trans-Himalayan High-Altitude Region.

Consumption of heavy metals contaminated dairy milk may affect human health. No such studies have been carried out at the high-altitude region to assess the human health risk after consuming dairy milk. To this end, this study reveals the evaluation of selected heavy metals in dairy milk in the summer and winter seasons, followed by the assessment of heavy metals health risk.

Basic chronobiology: what do sleep physicians need to know?

Sleep is an essential physiological process, which profoundly affects a wide range of biological activities. It is now known that sleep supports myriad vital functions in the central nervous system. This includes neural plasticity, learning, memory, cognition and emotional regulation.

Correction: One pot green preparation of silver nanoparticles (SBT@AgNPs) featuring high stability and longevity, antibacterial, antioxidant potential: a nano disinfectant future perspective.

[This corrects the article DOI: 10.1039/C7RA10262C.].

Correction: Synthesis of non-toxic, biocompatible, and colloidal stable silver nanoparticle using egg-white protein as capping and reducing agents for sustainable antibacterial application.

[This corrects the article DOI: 10.1039/C8RA03649G.].

Morphometric, haematological and physio-biochemical characterization of Bactrian (Camelus bactrianus) camel at high altitude.

Background: Biochemical and haematological parameters have not been determined in Bactrian camels kept at high altitude. Therefore, this study was undertaken to characterise different physiological, haematological, biochemical, and morphometric parameters of Bactrian camels of high altitude. For this, total fourteen high altitude healthy Bactrian camels were selected from Leh-Ladakh, India, a high altitude area, and thereafter divided into three age groups (N = 3 young; N = 6 adult; N = 5 old camels) to characterise for above parameters.

Antibiofilm potential of Seabuckthorn silver nanoparticles (SBT@AgNPs) against .

In era of antibiotic resistance, antibacterial silver nanoparticles are considered as potential alternative therapeutic agent to combat drug resistant pathogens. The aim of present study was to evaluate the antibacterial, antibiofilm and biocompatible potential of green synthesized Seabuckthorn silver nanoparticles (SBT@AgNPs). In the study, antibacterial efficiency of SBT@AgNPs was studied against , , and methicillin resistant .

Chemometric Analysis of Antioxidant and Mineral Elements in Colostrum of Native and Non-native Goat Breeds to Hypoxic Conditions at High Altitude.

Colostrum of goat is a well-known nutritional source of animal product, which is attributed to innumerable nutritional properties. To enrich nutritional resources for understanding various nutritional values of animal product at high altitude, chemometric analysis of antioxidant and mineral element study was carried out by comparing antioxidants capacity, free radical scavenging activity, and certain mineral elements in colostrums of native and non-native goat breeds. Colostrum samples were collected from native Changthangi (CNG) and non-native Sirohi (SIRO) goat breeds, situated at naturally exposed high altitude of 3505.

Chronotherapeutics: Recognizing the Importance of Timing Factors in the Treatment of Disease and Sleep Disorders.

This review describes the characteristics of a number of pathologies, which are considered from the point of view of chronobiology, that is, the way in which biological processes are expressed throughout the 24-hour day. This perspective is a relatively new way of thinking about disease and additionally about how to treat diseases. It has called attention to the importance of not only the quantity of a drug that is administered but also when it is administered.

Synthesis of non-toxic, biocompatible, and colloidal stable silver nanoparticle using egg-white protein as capping and reducing agents for sustainable antibacterial application.

For nearly a decade, silver nanoparticles (AgNPs) have been the most prevalent commercial nanomaterials products widely used in different biomedical applications due to their broad-spectrum antimicrobial activity. However, their poor long-term stability in different environments, namely, pH, ionic strength, and temperature, and cytotoxicity toward mammalian cells has restricted their more extensive applications. Hence, there is urgent need to develop highly biocompatible, non-toxic, and stable silver nanoparticles for wide-ranging environments and applications.

Overexpression of genes associated with hypoxia in cattle adapted to Trans Himalayan region of Ladakh.

Ladakh is an important part of the Trans-Himalayan region located between the Kunlun mountain range in the north and the main Great Himalayas to the south in the state of Jammu and Kashmir of India. The local cattle from Leh and Ladakh region, known as "Ladakhi cattle" is a unique germplasm having an excellent adaptation potential to high altitude hypobaric stress. In the present study, an effort was made to evaluate the transcriptional pattern of hypoxia inducing factor-1 (HIF-1) and several of its regulated genes in PBMCs of local Ladakhi cattle, Holstein Frisian crosses, Jersey (exotic) maintained at high altitude region and Sahiwal (Bos indicus) and Karan Fries (cross bred) cattle maintained in tropical environment.

Hippophae rhamnoides as novel phytogenic feed additive for broiler chickens at high altitude cold desert.

Extremes of climate and hypobaric hypoxia cause poor growth performance in broiler chickens at high altitude. The present study examined the potential of Hippophae rhamnoides extract as phytogenic feed additive for broilers reared at 3500 m above mean sea level (MSL). Higher content of phytomolecules were recorded during characterization of the extract.

Effect of seed extract on health, survivability, antioxidant, blood biochemical and immune status of broiler chickens at high altitude cold desert.

Extreme climatic conditions and hypobaric hypoxia at high altitude hinders the growth and productivity of chickens. The present study was carried out to examine the effect of aqueous extract of seeds on health, survivability, antioxidants, plasma biochemical parameters, and immune status of broiler chickens at high altitude. Phytochemical analysis of extract revealed the presence of high phenolics, flavonoids, and carotenoids contents.

Studies on the growth performance of different broiler strains at high altitude and evaluation of probiotic effect on their survivability.

Identification of appropriate breeds of broilers and development of new feed additives is required for the development of poultry industry at high altitude. Therefore, this experiment was conducted first to identify the suitable broiler strain for this region. One week old chicks (150) from three broiler strains, i.

Sequence analysis of the Toll-like receptor 2 gene of old world camels.

The Toll-like receptor 2 (TLR2) gene of old world camels (Camelus dromedarius and Camelus bactrianus) was cloned and sequenced. The TLR2 gene of the dromedary camel had the highest nucleotide and amino acid identity with pig, i.e.

Effects of melatonin and epiphyseal proteins on fluoride-induced adverse changes in antioxidant status of heart, liver, and kidney of rats.

Several experimental and clinical reports indicated the oxidative stress-mediated adverse changes in vital organs of human and animal in fluoride (F) toxicity. Therefore, the present study was undertaken to evaluate the therapeutic effect of buffalo (Bubalus bubalis) epiphyseal (pineal) proteins (BEP) and melatonin (MEL) against F-induced oxidative stress in heart, liver, and kidney of experimental adult female rats. To accomplish this experimental objective, twenty-four adult female Wistar rats (123-143 g body weights) were divided into four groups, namely, control, F, F + BEP, and F + MEL and were administered sodium fluoride (NaF, 150 ppm elemental F in drinking water), MEL (10 mg/kg BW, i.

Melatonin antioxidative defense: therapeutical implications for aging and neurodegenerative processes.

The pineal product melatonin has remarkable antioxidant properties. It is secreted during darkness and plays a key role in various physiological responses including regulation of circadian rhythms, sleep homeostasis, retinal neuromodulation, and vasomotor responses. It scavenges hydroxyl, carbonate, and various organic radicals as well as a number of reactive nitrogen species.

Evaluation of blood antioxidant defense and apoptosis in peripheral lymphocytes on exogenous administration of pineal proteins and melatonin in rats.

In view of the significant health impact of oxidative stress and apoptosis dysfunction, and further, because of suggestions that administration of antioxidants might reduce apoptosis rate through up-regulation of body antioxidant defense systems, therefore the purpose of this study was to compare the effect of buffalo (Bubalus bubalis) pineal proteins (PP at 100 μg/kg BW, i.p.) with melatonin (MEL at 10 mg/kg BW, i.