An accurate and reliable forecast of biosurfactant production with minimum error is useful in any bioprocess engineering. Bacterial isolate FKOD36 capable of producing biosurfactant was isolated in this study and pre-inoculums was prepared from the agar slants in a small test tube and incubated at 30 °C for 24 h at 120 rpm. Due to inherent non-linearity characteristics of the data set in a bioprocess, conventional modeling techniques are not adequate for predicting biosurfactant production in a microbiological process.
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