infection in threatened Indian orchid mobilises 'Moscatilin' to enhance plant defensins.

Unlabelled: The present study illustrates the transformation ability of two wild-type bacterial strains of s (MTCC 532 and MTCC 2364) on the embryogenic callus and callus-derived plantlets of a threatened Indian orchid, . Co-culture of the bacterium with the explants gave marginal hairy root phenotype that failed to multiply in the culture medium. Some primary and secondary metabolites were subdued in infected explants.

In vitro bioproduction and enhancement of moscatilin from a threatened tropical epiphytic orchid, (Willd.) Kraenzl.

Moscatilin, a bibenzyl derivative (stilbenoid), mostly found in one of the largest genera of Orchidaceae; has many therapeutic benefits. Its function as an anticancer agent has been widely demonstrated through many research investigations. However, the compound has not been produced in vitro to date.

Docking and ADMET studies for investigating the anticancer potency of Moscatilin on APC10/DOC1 and PKM2 against five clinical drugs.

Background: Moscatilin is a bibenzyl derivative (stilbenoid), mainly found in Dendrobium species. This plant-derived chemical is a potential cytotoxic anticancer drug that acts against different cancer types. The present study compared the structural interactions of Moscatilin along with five clinically relevant drugs against two target proteins, viz.

Cytotoxicity and radiosensitizing potency of Moscatilin in cancer cells at low radiation doses of X-ray and UV-C.

Unlabelled: Moscatilin (stilbenoid) is a plant-derived anticancer compound, and it has mostly been isolated from threatened wild species. The present study attempts to evaluate the cytotoxicity of Moscatilin on several cancer cell lines through MTT assay. Additionally, it also aims towards estimating and comparing the radiosensitivity, cell-cycle progression, and apoptotic/necrotic effect induced by Moscatilin on different cell lines.

Native and non-native host assessment towards metabolic pathway reconstructions of plant natural products.

Plant-based biopreparations are reasonably priced and are devoid of viral, prion and endotoxin contaminants. However, synthesizing these natural plant products by chemical methods is quite expensive. The structural complexity of plant-derived natural products poses a challenge for chemical synthesis at a commercial scale.

Cell size: a key determinant of meristematic potential in plant protoplasts.

Metabolic pathway reconstruction and gene edits for native natural product synthesis in single plant cells are considered to be less complicated when compared to the production of non-native metabolites. Being an efficient eukaryotic system, plants encompass suitable post-translational modifications. However, slow cell division rate and heterogeneous nature is an impediment for consistent product retrieval from plant cells.

Dendrobium protoplast co-culture promotes phytochemical assemblage in vitro.

The present study is intended to analyze the occurrence of potent, low produce, naturally occurring stilbenes in protoplasts of wild species and hybrids of Dendrobium. The wild species selected for the study was Dendrobium ovatum, endemic to Western Ghats of India. Protoplasts were isolated from leaves and tepal tissues of all the species and were cultured purely to generate homofusants and cross-cultured to raise heterofusants.

Influence of helium-neon laser irradiation on seed germination in vitro and physico-biochemical characters in seedlings of brinjal (Solanum melongena L.) var. Mattu Gulla.

In the present study, the seeds of brinjal (Solanum melongena L.) var. Mattu Gulla were irradiated with single exposure of He-Ne laser at different doses of 5-40 J cm(-2) and germinated aseptically.

Rapid detection and characterization of Chikungunya virus by RT-PCR in febrile patients from Kerala, India.

There has been a resurgence and prevalence of fever with symptoms of Chikungunya (CHIK) and increased death toll in Kerala, the southern-most state of India. The objective of this study was to develop a rapid detection method to determine the presence of CHIK- virus in the serum samples collected from febrile patients in Kerala, India. Serum specimens were analyzed for CHIK viral RNA by RT-PCR using primers specific for nsP1 and E1 genes.