A genome-wide overexpression screen reveals Mycobacterium smegmatis growth inhibitors encoded by mycobacteriophage Hammy.

During infection, bacteriophages produce diverse gene products to overcome bacterial antiphage defenses, to outcompete other phages, and to take over cellular processes. Even in the best-studied model phages, the roles of most phage-encoded gene products are unknown, and the phage population represents a largely untapped reservoir of novel gene functions. Considering the sheer size of this population, experimental screening methods are needed to sort through the enormous collection of available sequences and identify gene products that can modulate bacterial behavior for downstream functional characterization.

Gentamicin-induced readthrough of stop codons in Duchenne muscular dystrophy.

Objective: The objective of this study was to establish the feasibility of long-term gentamicin dosing to achieve stop codon readthrough and produce full-length dystrophin. Mutation suppression of stop codons, successfully achieved in the mdx mouse using gentamicin, represents an important evolving treatment strategy in Duchenne muscular dystrophy (DMD).

Methods: Two DMD cohorts received 14-day gentamicin (7.

Balancing the risk of chronic gastrointestinal bleeding and thromboembolic events in a patient with a metallic aortic valve.

Evidence shows clear benefit of anticoagulation for prosthetic heart valves but consideration must be given to coexisting medical conditions.