The gene product of a Trypanosoma equiperdum ortholog of the cAMP-dependent protein kinase regulatory subunit is a monomeric protein that is not capable of binding cyclic nucleotides.

The full gene sequence encoding for the Trypanosoma equiperdum ortholog of the cAMP-dependent protein kinase (PKA) regulatory (R) subunits was cloned. A poly-His tagged construct was generated [TeqR-like(His)], and the protein was expressed in bacteria and purified to homogeneity. The size of the purified TeqR-like(His) was determined to be ∼57,000 Da by molecular exclusion chromatography indicating that the parasite protein is a monomer.