Publications by authors named "Victoria F Beja-Glasser"
Article Synopsis
- Researchers examined over 2.4 million brain cells from 18 locations in the common marmoset using single-nucleus RNA sequencing to analyze gene expression patterns in various brain structures.
- The findings suggest that the adult identity of most neuron types is influenced more by their developmental origins than by the types of neurotransmitters they release.
- High proportions of specific neuron types were found in higher-order cortical areas, and the study utilized cell type-specific enhancers and AAV-GFP to visualize different interneuron morphologies in the neocortex and striatum.
Unbiased in vivo genome-wide genetic screening is a powerful approach to elucidate new molecular mechanisms, but such screening has not been possible to perform in the mammalian central nervous system (CNS). Here, we report the results of the first genome-wide genetic screens in the CNS using both short hairpin RNA (shRNA) and CRISPR libraries. Our screens identify many classes of CNS neuronal essential genes and demonstrate that CNS neurons are particularly sensitive not only to perturbations to synaptic processes but also autophagy, proteostasis, mRNA processing, and mitochondrial function.
View Article and Find Full Text PDFSynapse loss in Alzheimer's disease (AD) correlates with cognitive decline. Involvement of microglia and complement in AD has been attributed to neuroinflammation, prominent late in disease. Here we show in mouse models that complement and microglia mediate synaptic loss early in AD.
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