We describe the development of a simple and highly sensitive electrochemical (amperometric) sensing of bilirubin based on bilirubin oxidase (BOx) incorporated into the gold nanoparticles (AuNPs). This nanoelectrode platform with self-assembled enzyme is highly sensitive toward the electrochemical oxidation of bilirubin and increased the bilirubin concentration linearly from 1 to 5000 μM with a correlation coefficient of 0.9960, and an apparent Michaelis constant (K(M,app)) of 44 ± 0.
View Article and Find Full Text PDFIntroduction: A higher rate of conversion to open surgery is a well-known problem in patients with acute cholecystitis undergoing laparoscopic cholecystectomy. The aim of this study is to analyze factors which may impact on conversion rates, and to analyze our outcomes following implementation of a departmental strategy in reducing conversion rates.
Materials And Methods: 122 patients with acute cholecystitis were considered for laparoscopic cholecystectomy from July 2003 to July 2007.