Modifying spiking precision in conductance-based neuronal models.

The temporal precision of a neuron's spiking can be characterized by calculating its "jitter," defined as the standard deviation of the timing of individual spikes in response to repeated presentations of a stimulus. Sub-millisecond jitters have been measured for neurons in a variety of experimental systems and appear to be functionally important in some instances. We have investigated how modifying a neuron's maximal conductances affects jitter using the leaky integrate-and-fire (LIF) model and an eight-conductance Hodgkin-Huxley type (HH8) model.

Laminar circuit organization and response modulation in mouse visual cortex.

The mouse has become an increasingly important animal model for visual system studies, but few studies have investigated local functional circuit organization of mouse visual cortex. Here we used our newly developed mapping technique combining laser scanning photostimulation (LSPS) with fast voltage-sensitive dye (VSD) imaging to examine the spatial organization and temporal dynamics of laminar circuit responses in living slice preparations of mouse primary visual cortex (V1). During experiments, LSPS using caged glutamate provided spatially restricted neuronal activation in a specific cortical layer, and evoked responses from the stimulated layer to its functionally connected regions were detected by VSD imaging.