Publications by authors named "Victor Lao"
Article Synopsis
- Transgenerational epigenetic inheritance (TEI) allows the passing of information across generations without altering DNA sequences, primarily through non-coding RNAs and chromatin changes.
- In Caenorhabditis elegans, RNA interference (RNAi) serves as a key model to study TEI, as gene silencing effects can be inherited for multiple generations after initial exposure.
- The described protocol utilizes a GFP reporter in C. elegans to analyze RNAi inheritance, encompassing feeding strategies, developmental synchronization, microscopy for gene silencing assessment, and techniques like ChIP-qPCR to examine histone modifications at specific gene loci.
