Robustifying Experimental Tracer Design forC-Metabolic Flux Analysis.

C metabolic flux analysis (MFA) has become an indispensable tool to measure metabolic reaction rates (fluxes) in living organisms, having an increasingly diverse range of applications. Here, the choice of theC labeled tracer composition makes the difference between an information-rich experiment and an experiment with only limited insights. To improve the chances for an informative labeling experiment, optimal experimental design approaches have been devised forC-MFA, all relying on some a priori knowledge about the actual fluxes.