Improved Protoplast Production Protocol for Fungal Transformations Mediated by CRISPR/Cas9 in Non-Sporulating Isolates.

is a necrotrophic fungus that causes considerable economic losses in commercial crops. Fungi of the genus exhibit great morphological and genetic variability, ranging from non-sporogenic and non-infective isolates to highly virulent sporogenic ones. There is growing interest in the different isolates in terms of their methodological applications aimed at gaining a deeper understanding of the biology of these fungal species for more efficient control of the infections they cause.

Unravelling the Function of the Sesquiterpene Cyclase STC3 in the Lifecycle of .

The genome sequencing of supplies a general overview of the map of genes involved in secondary metabolite synthesis. genomic data reveals that this phytopathogenic fungus has seven sesquiterpene cyclase () genes that encode proteins involved in the farnesyl diphosphate cyclization. Three sesquiterpene cyclases (BcStc1, BcStc5 and BcStc7) are characterized, related to the biosynthesis of botrydial, abscisic acid and (+)-4-epi-eremophilenol, respectively.

Comparing Fungal Sensitivity to Isothiocyanate Products on Different spp.

Glucosinolates, the main secondary metabolites accumulated in cruciferous flora, have a major impact on fortifying plant immunity against diverse pathogens. Although exhibits varying sensitivity to these compounds, current research has yet to fully understand the intricate mechanisms governing its response to glucosinolates. Different species of the genus were exposed to glucosinolate-derived isothiocyanates, revealing that , , and , species with the transporter gene (Bcin06g00026) not detected with PCR, were more sensitive to isothiocyanates than species containing that gene, such as , , and .

From Genes to Molecules, Secondary Metabolism in : New Insights into Anamorphic and Teleomorphic Stages.

The ascomycete Pers. Fr., classified within the family Sclerotiniaceae, is the agent that causes grey mould disease which infects at least 1400 plant species, including crops of economic importance such as grapes and strawberries.

Structures, Occurrences and Biosynthesis of 11,12,13-Tri--Sesquiterpenes, an Intriguing Class of Bioactive Metabolites.

The compounds 11,12,13-tri--sesquiterpenes are degraded sesquiterpenoids which have lost the C unit of isopropyl or isopropenyl at C-7 of the sesquiterpene skeleton. The irregular C-backbone originates from the oxidative removal of a C side chain from the C sesquiterpene, which arises from farnesyl diphosphate (FDP). The C-framework is generated, generally, in all families of sesquiterpenes by oxidative cleavage of the C substituent, with the simultaneous introduction of a double bond.