Publications by authors named "Victor Chinomso Ujor"
Article Synopsis
- The study developed hydrophobic stainless steel meshes for effective recovery of ABE (acetone, butanol, and ethanol) from bioreactors during vacuum-assisted gas stripping, minimizing water loss.
- Three types of meshes with varying pore sizes (180 µm, 300 µm, and 425 µm) were coated with zinc oxide and polydimethylsiloxane, showing that the 180-µm mesh retained significantly more water (54-65% more) compared to uncoated ones.
- The technology increased butanol concentration in recoveries by up to 10.8-fold and proved effective for real-time ABE extraction from lignocellulosic fermentation without clogging issues.
The presence of lignocellulose-derived microbial inhibitory compounds (LDMICs) in lignocellulosic biomass (LB) hydrolysates is a barrier to efficient conversion of LB hydrolysates to fuels and chemicals by fermenting microorganisms. Results from this study provide convincing evidence regarding the effectiveness of metabolically engineered NCIMB 8052 for the fermentation of LB-derived hydrolysates to acetone-butanol-ethanol (ABE). The engineered microbial strain (_SDR) was produced by the integration of an additional copy of a short-chain dehydrogenase/reductase (SDR) gene (3904) into the chromosome of NCIMB 8052 wildtype, where it is controlled by the constitutive thiolase promoter.
View Article and Find Full Text PDFCarbon catabolite repression (CCR) limits microbial utilization of lignocellulose-derived pentoses. To relieve CCR in NCIMB 8052, we sought to downregulate catabolite control protein A (CcpA) using the M1GS ribozyme technology. A CcpA-specific ribozyme was constructed by tethering the catalytic subunit of RNase P (M1 RNA) to a guide sequence (GS) targeting CcpA mRNA (M1GS).
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