Publications by authors named "Vibhu Chandrashekhar"
Mapping cranial vasculature and adjacent neurovascular interfaces in their entirety will enhance our understanding of central nervous system function in any physiologic state. We present a workflow to visualize in situ murine vasculature and surrounding cranial structures using terminal polymer casting of vessels, iterative sample processing and image acquisition, and automated image registration and processing. While this method does not obtain dynamic imaging due to mouse sacrifice, these studies can be performed before sacrifice and processed with other acquired images.
View Article and Find Full Text PDFUnderstanding physiologic and pathologic central nervous system function depends on our ability to map the entire cranial vasculature and neurovascular interfaces. To accomplish this, we developed a non-invasive workflow to visualize murine cranial vasculature via polymer casting of vessels, iterative sample processing and micro-computed tomography, and automatic deformable image registration, feature extraction, and visualization. This methodology is applicable to any tissue and allows rapid exploration of normal and altered pathologic states.
View Article and Find Full Text PDFArticle Synopsis
- Researchers have discovered a previously unknown transtentorial venous system (TTVS) that connects venous drainage in the human brain, challenging earlier beliefs that this part was mainly avascular.
- The study used mice as a model to investigate the TTVS and found that it is present in adult mice and can be traced back to an embryonic stage where it aids in draining the choroid plexus.
- The findings suggest that the TTVS is conserved between humans and mice, making mice a suitable model for further research into the development and implications of this venous system in brain physiology.
Article Synopsis
- The inner ear, located within the petrous bone of the skull, plays a critical role in transmitting sound and balance signals to the brain through cranial nerves, developing from neural crest during early growth stages.
- While the anatomy of the inner ear in mice has been well-studied only up to day 6 after birth, both mice and humans continue to develop their inner ear and skull base into early adulthood.
- Traditional methods for studying the inner ear structure have limitations, particularly as the bone hardens after birth, but modern imaging techniques like high-resolution Micro-CT and MRI now enable detailed 3D visualization of this complex anatomy, resulting in the creation of a new atlas for the murine inner ear.