Optimized Biobanking Procedures for Preservation of RNA in Tissue: Comparison of Snap-Freezing and RNAlater-Fixation Methods.

Personalized treatment, supported by biomarkers, would improve survival of ovarian cancer patients. RNA molecules are potentially important biomarkers. The Danish CancerBiobank provides an infrastructure for handling and storage of biological material, including RNA, from Danish cancer patients.

Glycodelin in endometrial flushing fluid and endometrial biopsies from infertile and fertile women.

Objective: To investigate in the natural cycle just before IVF, whether glycodelin levels in endometrial flushing fluid obtained days LH+1 and LH+7 can be used in predicting pregnancy in the following IVF cycle, and whether there are differences in women with tubal factor infertility compared to women with unexplained infertility and fertile controls.

Study Design: A prospective observational multicentre study of 21 fertile and 75 infertile women (25 showed abnormal tubes with no signs of hydrosalpinges, 18 had uni- or bi-lateral hydrosalpinges, 17 were salpingectomised because of hydrosalpinges, and 15 women had unexplained infertility). Endometrial flushing at days LH+1 and LH+7, endometrial biopsy, and blood sampling at day LH+7 were performed before down-regulation for IVF.

Ultrasound characteristics and histological dating of the endometrium in a natural cycle in infertile women compared with fertile controls.

Objective: To compare endometrial dating and ultrasound texture in a natural cycle before IVF and relate these to outcome and to fertile references.

Design: Prospective study with a fertile reference group.

Setting: Four university IVF clinics.

The antiprogesterone Org 31710 inhibits human blastocyst-endometrial interactions in vitro.

Objective: To investigate the effect of the anti-P Org 31710 on human blastocyst attachment to cultured endometrial epithelial cells.

Design: Experimental in vitro study.

Setting: University hospital.

Biochemical evaluation of endometrial function at the time of implantation.

Objective: To review the literature on various endometrial factors assumed to be of importance to implantation and to evaluate their potential clinical value in the assessment of endometrial function at the time of implantation in infertile women in natural and stimulated cycles.

Design: Literature review.

Result(s): Cytokines such as leukemia inhibitory factor, colony-stimulating factor-1, and interleukin-1 have all been shown to play important roles in the cascade of events that leads to implantation.