[Differential expression of genes that encode glycolysis enzymes in kidney and lung cancer in humans].

Glycolysis is a main catabolic pathway of glucose metabolism, accompanied by ATP synthesis. More than 30 enzymes are involved in glycolysis, and genes that encode them can be considered housekeeping genes due to the high conservatism and evolutionary antiquity of the process. We studied the expression of these genes in kidney papillary cancer and planocellular lung cancer via the bioinformatic analysis of transcriptome database and method of quantitative real time PCR.

[Synthesis and acceptor properties of 11-[(9'-anthracenyl)methoxy]undecyl phosphate and P1-{11-[(9'-anthracenyl)methoxy]undecyl}-P2-(alpha-D-galactopyranosyl) diphosphate in the enzymic reactions catalyzed by galactosylphosphotransferase and mannosyltransferase from Salmonella newport].

Fluorescent 11-[(9'-anthracenyl)methoxy]undecyl phosphate and P1-{11-[(9'-anthracenyl)methoxy]undecyl}-P2-(alpha-D-galactopyranosyl) diphosphate were chemically synthesized for the first time. The ability of the first compound to serve as substrate-acceptor ofgalactosyl phosphate residue and the second compound of mannosyl residue in enzymic reactions catalyzed by galactosylphosphotransferase and mannosyltransferase from Salmonella newport membrane preparation was demonstrated.

[Synthesis of p(1)-(11-phenoxyundecyl)-P(2)-(alpha-D-galactopyranosyl) diphosphate and p(1)-(11-phenoxyundecyl)-P(2)-(alpha-D-glucopyranosyl) diphosphate; assay of their acceptor properties for mannosyl residue in the enzmic reaction catalyzed by mannosyltransferase from Salmonella newport].

P(1)-(Phenoxyundecyl)-P(2)-(alpha-D-galactopyranosyl) diphosphate as well as P(1)-(11-phenoxyundecyl)-P(2)-(alpha-D-glucopyranosyl) diphosphate are newly synthesized and their ability to serve as substrate-acceptor of mannosyl residue in enzymic reaction catalyzed by mannosyltransferase from Salmonella newport membrane preparation is investigated. The possibility ofgalactosyl-containing derivative to serve as mannosyl acceptor from GDP-Man is established whereas glucosyl-containing compound is inactive in this process.

[Possible mechanisms of aftereffects of GSM electromagnetic radiation on air-dry seeds].

Some physical treatments, such as microwave- and gamma-radiation and magnetic field, induce long-term transition of air-dry seeds from the fraction of strong seeds into the weak seed fraction, due to non-enzymatic hydrolysis ofbiomacromolecules. These physical factors make water molecules more active, which is followed by the release of water molecules from the hydration layer, disturbance of this layer structure, further activation of water molecules by means of the "domino effect," and accumulation of hydrolysis products.

[Non-thermal effect of GSM electromagnetic radiation on quality of pea seeds].

The seeds with low level of room temperature phosphorescence (RTP) were selected from a lot of air-dry peas (Pisum sativum) with 62% germination. These strong seeds (95-97% germination percentage) in air-dry, imbibed or emerged states were exposed to 905 MHz GSM-band electromagnetic radiation (EMR). The following effects of EMR were observed.

[Increase in NETO2 gene expression is a potential molecular genetic marker in renal and lung cancers].

Multiple changes in the genome, transcriptome, and proteome are frequent in cancer cells. A search for molecular markers based on DNA, mRNA, or proteins is a main method to develop early specific diagnostics for cancer. While universal markers are still unavailable, similar trends are known for the expression patterns of particular genes in certain epithelial tumors.

[Mechanism of injury of air-dry pea seeds under the influence of low doses of gamma-radiation].

The aim of this work was to determine which processes in air-dry seeds result in bimodal changes of the pea seed quality under the influence of low doses of gamma-radiation. Pea seeds (cv. "Nemchinovsky-85", harvest 2006, 82% germination persentage) were exposed to gamma-radiation at doses of 3, 10 and 100 Gy The germination percentage decreased to 45% four days after irradiation at the dose of 3 Gy, rised up to 87% at doses of 10 Gy, while the dose of 100 Gy killed the most part of seeds.

[Simple synthesis of P¹-(11-phenoxyundecyl)-P²-(2-acetamido-2-deoxy-α-D-galactopyranosyl) diphosphate].

A simple scheme of synthesis of P¹-(11-phenoxyundecyl)-P²-(2-acetamido-2-deoxy-α-D-galactopyranosyl) diphosphate synthetic lipid acceptor for glycosyltransferases participating in gram-negative bacteria O-antigenic polysaccharides is suggested.

[Synthesis of 11-phenoxyundecyl phosphate and its use as an acceptor substrate in the reaction with UDP-GlcNAc : polyprenyl phosphate GlcNAc-phosphotransferase from Salmonella arizona O:59].

A new scheme of synthesis of 11-phenoxyundecyl phosphate from 11-bromoundecanoic acid was suggested for its ability to react as an acceptor of 2-acetamido-2-deoxy-alpha-D-glucopyranosyl phosphate in a reaction catalyzed by UDP-N-acetylglucosamine : polyprenyl phosphate N-acetylglucosamine phosphotransferase from Salmonella arizona O:59.

[The disruption of aquaporin function in cell membranes as a cause of changes in germinability of pea seeds after exposure to low doses of gamma-radiation].

Pea seeds (Pisum sativum L.) from the seed lot with 80% germinability were separated in fractions according to room temperature phosphorescence: strong seeds were assigned to fraction I, and weak seeds formed to fraction II. During imbibition, the seeds of fraction II exhibited twofold higher rates of water uptake and experienced hypoxia.

[Bimodal changes in germinability of pea seeds under the influence of low doses of gamma-radiation].

Pea seeds (cv. 'Nemchinovskii-85', harvest of 2002, 80%-germination percentage) were exposed to gamma-radiation with doses ranging from 19 cGy to 100 Gy. One week after the irradiation with doses of 19 cGy and 3 Gy.

[A case of deletion of the short arm of the chromosome 21 (21p-) (christchurch chromosome) discovered prenatally:clinical and cytogenetic data].

Results of cytogenetic research of placental villi and amniotic fluid cells culture of the 22-weeks-old fetus with multiple congenital malformations (MCM) are presented. The absence of the short arm in one of the homologue of the chromosome 21 was revealed. Cytogenetic analysis of the fetus father's blood lymphocytes determined the similar chromosome.

[Bimodal changes in the germination of seeds subjected to heat treatment].

Novel explanation is proposed for the improvement of low quality seeds by pre-sowing treatment with factors of various nature (heat and sonic treatment, electrical field of corona discharge, laser irradiation). The increase in seed germination percentage is caused by slowing-down of seed water uptake as a result of closing the aquaporin water canals.

[The study of the mixing layer by the point image method].

The properties of the mixing layer in dynamic systems were studied by the example of a mathematical model of the cubic image type. Its role in the generation of information and the evolution of its significance was shown. At the moment of generation, information is of zero significance, and this significance then increases.

[The necessity of taking into account sanogenetic and pathogenetic reactions in the treatment of patients with the neurodystrophic form of lumboischialgia].

The authors have analyzed the results of clinico-instrumental examination of patients with a neurodystrophic form of lumbar ischialgia associated with lumbar osteochondrosis and specified two groups of symptoms: (1) due to pathogenetic manifestations of the disease and (2) due to sanogenetic reactions of the body.

[Meralgia paresthetica in patients with osteochondrosis of the spine].

Clinical and electrophysiologic examinations of 1220 patients with spinal osteochondrosis syndromes have made it possible to distinguish two variants of meralgia paresthetica, permanent and transitory ones. Different methods for the management of the syndrome were developed, with due consideration for the aforementioned variants.

[Myofixation in the patho- and sanogenesis of lumbar osteochondrosis].

Neuroorthopedic , electromyographic and neuropsychological examinations of patients with lumbar osteochondrosis have shown that dynamic myofixation in health is regulated by the preceding muscular reactions. When these mechanisms are impaired, the disease is characterized by an early onset and a severe course. With the development of osteochondrosis the myofixation in the damaged spinal segment is regulated by the reflex responses to the impulses from the zone of the damaged disk and from the focus of aseptic inflammation developing there rather than by the preceding muscular reactions.

[Prolonged pharmacologic sleep in the complex treatment of patients with severe forms of alcoholic delirium].

Results of treating 235 patients suffering from grave alcohol delirium complicated with somatic pathology are presented. Use was made of various methods of inducing prolonged drug sleep: by neuroleptics, barbiturates with analgesics sodium gamma-hydroxybutyrate alone or in combination with barbiturates or viadril. The method of using the combination of sodium gamma-hydroxybutyrate with barbiturates or viadril G appeared to be the most effective.