[Influence of professional conditions as risk factor for the development of vascular eye pathology in surgeons].

This work was devoted to study of the peculiarities of morphology of the retina with ganglion cells complex and the peculiarities of regional and systemic blood circulation in surgeons and therapists. It was revealed that longtime work under operative microscope results in a decrease of all parameters of blood circulation, that with the data of optical coherent tomography of the retina and optic nerve in surgeons demonstrate the signs of the existence of more high risk of vascular eye pathology then that observed in therapists.

Mutation screening of the BRCA1 gene in Slovak patients.

Breast cancer is the most commonly observed malignancy in women of the western world. The family history is the strongest risk factor for the disease. Two major genes, BRCA1 and BRCA2 that are involved in the familial breast and ovarian cancer have been described.

[Bioactivity and effect of a mixture of pilocarpine and lysine on the pupil and intraocular pressure in rabbits].

Based on experiments with rabbit eyes (strain New Zealand white) the authors submit evidence that the metabolite which is formed as a result of interaction of 2% pilocarpine and L-lysine 2 HCl.2H2O has a significantly greater and more rapid effect on the development of miosis than 2% pilocarpine alone. The amino acid lysine does not have a miotic effect.

[Effect of pilocarpine in tissue structures of the anterior eye segment from the aspect of its interaction with free amino acids].

On the basis of the proofs of pilocarpine interaction with free amino acids, the mechanism of this alkaloid effect in the tissue structures of the eye anterior segment is explained. Differences of colour reactions of amino acids mixtures with pilocarpine, decrease of radioactivity of Na[I125]tyrosine mixture with pilocarpine, unsteadiness of radioactivity of Na[I125]protein A mixture with pilocarpine and rise of 16 radioactivity fractions after adding pilocarpine to Na[I125]proteins A a) show the specificity of interaction of each amino acid with pilocarpine; b) give evidence of a strong covalent bond between pilocarpine and free amino acid and give rise to new biologically active metabolite; c) show non-cholinergic pathway of pilocarpine effect; d) for ophthalmo-physiological practice it is said that with respect to the rate between pilocarpine quantity and concentration and free amino acid quantity and concentration requested therapeutical effect by lower pilocarpine dose in glaucoma disease treatment might be achieved. Thus free amino acids being reactants can be identified as mechanism by which pilocarpine becomes biologically active.

Immunosuppressive activity of lymphocyte mitogenesis by breast cancer-associated p43.

Placental isoferritin (PLF), an acidic isoform of ferritin, and its unique superheavy chain of 43 kDa (p43) has been described to be synthesized by human breast cancer cells. Physiologically, p43 PLF produced by the placenta is involved in immune suppression of maternal lymphocytes aimed at fetal antigens. A study was carried out to elucidate a paradigm of p43 occurrence in breast cancer patients.

Expression of p43 associated placental isoferritin (PLF) correlates inversely with cell growth in breast cancer cell lines MCF-7 and T47-D.

We have previously demonstrated that the expression of the recently described immunosuppressive antigen p43 in breast cancer patients correlates with early stages of the disease and a low degree of proliferation of the tumors. Attempts were made to evaluate the expression of p43 in two breast cancer cell lines (MCF-7 and T47-D) stimulated to proliferation by 17-beta estradiol and fetal bovine serum (FBS). p43 expression was determined by RIA technique using the new monoclonal antibody CM-H-9, the rate of proliferation was assessed by [3H]thymidine incorporation during 72 hours of incubation.

Comparison of two defective hepatitis A virus strains adapted to cell cultures.

The replication of two defective hepatitis A virus strains in cell culture was examined. The w.t.

Radioimmunoscintigraphy of human malignant melanoma. I. Pharmacokinetics and localization of 125I-labeled monoclonal antibody RG-12 in thymectomized mice bearing renal melanoma xenotransplants.

The novel RG-12 monoclonal antibody (MoAb) recognizing a high-molecular-weight antigen of human melanoma cells was radioiodinated and its biodistribution and tumor imaging was determined in immunosuppressed mice bearing xenografted human malignant melanoma HMB-2. Control and tumor-bearing mice were injected with 6 micrograms of 125I-labeled RG-12 IgG (8.9 MBq 125I-IgG/animal).

Morphologic and antigenic properties of mouse mammary tumor virus produced in a hormone-responsive fashion by C57Bl/10 mammary tumors of non-viral origin.

Bl-MaTU/A1 mouse mammary tumor cells, derived from a C57B1/10 mammary adenocarcinoma induced by dimethylbenzanthracene and mammotropic hormones, express virus particles and proteins related to mouse mammary tumor virus (MMTV). Immunocytochemical analysis by means of monospecific and monoclonal anti-gp52 sera revealed a different localization of the main structural proteins of MMTV in Bl-MaTU/A1 and GR cells (the latter used as a positive virus-producing control). Immunoelectron microscopy of B-type particles budding from the microvilli of dexamethasone-stimulated Bl-MaTU/A1 cells showed remarkably weak reactivity of the viral envelope with anti-gp52-protein A-gold complexes as compared with that of dexamethasone-stimulated GR cells.

Biological and immunological properties of the HMB-2 human melanoma cell line.

The HMB-2 human melanoma cell line derived from a lymph node metastatis is described. After long-term cultivation in vitro the cells retained their morphology, high growth rate, xenotransplantability into immunosuppressed mice and genotypic and phenotypic markers of human melanoma cells. Upon infection of the HMB-2 cells with temperature-sensitive mutant vesicular stomatitis virus (VSVts045) at nonpermissive temperature, a complemented virus pseudotype (VSV(HMB-2] was produced carrying assembled melanoma-associated antigens.

[Expression of virus-specific functions in 3-methylcholanthrene-induced leukemia in rats].

It was isolated and identified MR-Le virus from Wistar rats with myelogenous leukemia. The comparison of isolated in vivo and in vitro MR-Le virus with endogenous oncovirus RaLV in protein profile as well as results of molecular hybridization pointed out that RaLV did not participate in the induction of MR leukemia. The question about the role of MR-Le virus in the etiology of MR leukemia is opened now.

Expression of retrovirus-related functions in the methylcholanthrene-induced rat myelogenous leukemia.

The rat MR-leukemia (MR-Le) induced in Wistar rats by methylcholanthrene and whole-body irradiation, has been shown to be transmitted by means of cell-free filtrates of spleen and liver extracts. These earlier results lead us to determine the expression of retroviral functions by MR-Le myeloblasts in vivo and in vitro. DNA polymerase activity associated with particulate material purified from plasma of rats carrying MR-Le and from MR-Le tissue culture fluid, increased with the number of MR-Le myeloblasts.