Differential activation of adenylate cyclase by secretin and VIP receptors in the calf pancreas.

Objective: Secretin is a key regulator of pancreatic secretion, but the molecular basis of its action is not well understood, especially in the calf pancreas. Our study investigated the expression and functional competence of secretin receptors (SEC-R) in calf pancreatic membranes.

Methods: We used reverse transcriptase-polymerase chain reaction, sequencing, and Northern blot to assess the expression of the SEC-R gene.