Probe-based qPCR as an alternative to modified Knott's test when screening dogs for heartworm (Dirofilaria immitis) infection in combination with antigen detection tests.

Background: Current recommendations for diagnosis of Dirofilaria immitis infection in dogs rely on the detection of antigen produced largely by adult females coupled with the visualization of microfilariae (mf) in the circulation via a microfilaria detection test (MFDT). It is hypothesized that qPCR assays used in parallel with antigen detection tests will perform better in detecting mf than modified Knott's test (MK), when combined with antigen detection. This study compares probe-based qPCR and MK techniques for mf detection used in parallel with the DiroCHEK antigen test to screen for heartworm infection in shelter dogs.

Detection of via integrated serological and molecular analyses in coyotes from Texas, United States.

Wild canids serve as reservoir for various vector-borne pathogens of veterinary and medical importance, including the canine heartworm, . In North and Central America, coyotes () may be a relevant reservoir host for heartworm transmission. The objective of this study was to determine the occurrence of in coyotes across Texas using integrated antigen detection test and molecular assays.

Association between vitamin D levels and blood pressure in a group of Puerto Ricans.

Objective: Hypertension is a global public health problem. The apparent association between vitamin D (VitD) and blood pressure (BP) has been studied in several populations but not in a Puerto Rican population. Therefore, we determined the association between serum 25(OH)D levels and BP in a convenience sample in Puerto Rico.