Results Multi-Laboratory Trial ISO/CD 22184-IDF/WD 244: Milk and Milk Products-Determination of the Sugar Contents-High-Performance Anion Exchange Chromatography Method with Pulsed Amperometric Detection.

Background: In this international Multi-Laboratory Trial (MLT) the precision data and the accuracy of the ISO/CD 22184 IDF/WD 244 analytical method for the quantification of 6 different mono- and disaccharides (galactose, glucose, fructose, sucrose, lactose, and maltose) in milk and milk products with high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) are established.

Objective: Thirteen different laboratories participated (located in New Zealand, the United States, and Europe). The study was divided into two parts.

Results Multi-Laboratory Trial ISO/CD 22184 - IDF/WD 244: Milk and milk products - Determination of the sugar contents - High performance anion exchange chromatography method with pulsed amperometric detection (HPAEC-PAD).

Background: In this international Multi Laboratory Trial (MLT) the precision data and the accuracy of the ISO/CD 22184 IDF/WD 244 analytical method for the quantification of 6 different mono- and disaccharides (galactose, glucose, fructose, sucrose, lactose and maltose) in milk and milk products with high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) are established.

Objective: Thirteen different laboratories participated (located in New Zealand, United States, Europe). The study was divided into two parts.

The Determination of Sugars in Dairy Products: Development of a New Standard Method for the International Dairy Federation and the Internal Organization for Standardization.

A method using high-performance anion-exchange chromatography (HPAEC) with a pulsed amperometric detector (PAD) for the determination of mono- and disaccharides is described. The method was accepted by the International Dairy Federation and the Internal Organization for Standardization as a new work item for the determination of sugars in dairy matrixes, and the Milk and Milk Products technical committee of ISO/TC 34/SC 5 accepted the topic "Milk and milk products - Determination of the sugar contents - High-performance anion-exchange chromatographic method (HPAEC-PAD)" as a new work item. The proposed method consists of an aqueous ethanol extraction of the sugars in the dairy sample, followed by clarification with Carrez I and II reagents.

Dual-Laboratory Validation of a Method for the Determination of Fructans in Infant Formula and Adult Nutritionals: First Action 2016.14.

Until recently, only two AOAC Official MethodsSM have been available for the analysis of fructans: Method 997.08 and Method 999.03.