The U5 snRNA internal loop 1 is a platform for Brr2, Snu114 and Prp8 protein binding during U5 snRNP assembly.

The U5 small nuclear ribonucleoprotein particle (snRNP) forms the heart of the spliceosome which is required for intron removal from pre-mRNA. The proteins Prp8, Snu114 and Brr2 all assemble with the U5 small nuclear RNA (snRNA) to produce the U5 snRNP. Successful assembly of the U5 snRNP, then incorporation of this snRNP into the U4/U6.

The role of Snu114p during pre-mRNA splicing.

Pre-mRNA splicing is an essential step in gene expression where intron regions are removed and coding exon sequences are joined to form an mRNA for translation. Splicing is catalysed by an RNA-protein complex called the spliceosome. A number of spliceosome proteins are required for assembly and remodelling of the spliceosome with pre-mRNA to orient the splice sites correctly and catalyse the two steps of splicing.