Publications by authors named "Vergara-Porras Berenice"

Article Synopsis
  • - Recombinant Cupriavidus necator H16/pMPJAS03 can create short/medium-chain-length (scl-mcl) PHA copolymers using a native enzyme and was grown on fructose combined with canola oil.
  • - The cells produced a polymer primarily made up of 3-hydroxybutyrate (3HB) and significantly higher amounts of 3-hydroxyoctanoate (3HO) and 3-hydroxydecanoate (3HD) compared to previous yields.
  • - Increasing the decanoic acid in the feed enhanced the production of 3HD and impacted the properties of the resulting polymers, demonstrating the potential for optimizing enzyme combinations to produce
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Initiatives to reduce plastic waste are currently under development worldwide. As a part of it, the European Union and private and public organizations in several countries are designing and implementing regulations for single-use plastics. For example, by 2030, plastic packaging and food containers must be reusable or recyclable.

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The acyl-CoA dehydrogenase (FadE) and (R)-specific enoyl-CoA hydratase (PhaJ) are functionally related to the degradation of fatty acids and the synthesis of polyhydroxyalkanoates (PHAs). To verify this, a recombinant Cupriavidus necator H16 harboring the plasmid -pMPJAS03- with fadE from Escherichia coli strain K12 and phaJ1 from Pseudomonas putida strain KT2440 under the arabinose promoter (araC-P) was constructed. The impact of co-expressing fadE and phaJ genes on C.

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Polyhydroxybutyrate (PHB) is biodegradable and biocompatible polyester that has been recently used for developing different drug delivery systems (DDS). Microspheres as DDS, consist of a polymeric matrix with a diameter of 1-125 μm which contains a substance entrapped, and then released mainly through diffusion. In order to make DDS viable for commercial applications, it is essential to develop models that describe and predict the substance release kinetic.

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