[The role of carrier-object in investigations of cigar-butts].

A comprehensive series of experiments was undertaken to find the reasons why the cigar-butt stuff, removed from accident places for investigation at forensic-biological laboratories, affects different sera. A number of such reasons were established. Methods are suggested to eliminate the cigar-butt impact with the aim of ensuring the most accurate results while determining the group adherence of saliva on the cigar-butts.

[Proliferative activity and cytotoxic effect of mouse lymphocytes during the growth of transplanted melanoma B-16].

The PHA stimulated proliferative activity of C57B1 mice lymphocytes and cell-mediated cytotoxicity on tumour target cells in vitro were studied during the transplantable melanoma B16 growth. The lymphocyte stimulation was shown to take place in all studied periods of the tumour growth with the exception of the terminal stage. The kinetics of cytotoxic activity during the melanoma B16 growth is characterized by rises and falls in the lymphocyte cytotoxicity.

[Correlation of the level of humoral cytotoxins and delayed hypersensitivity skin reactions with survival of patients with melanoma of the skin].

The study was concerned with relationships between blood-humoral cytotoxin level, time-delayed hypersensitivity skin reaction to DNCB and 3- and 5-year survival in skin melanoma patients. Humoral cytotoxin level proved to be irrelevant for prognosis, although this index may be sometimes used as a supplementary criterion for evaluating immunocompetence. Skin reactions to DNCB provide a valuable prognostic means in skin melanoma patients: 3- and 5-year survival rates are significantly higher in patients showing positive reaction than in those with negative reaction.

[Cytotoxic immunity indices of melanoma B16 growth in mice].

Lymphocytes and sera in mice with melanoma B16 and in intact mice render a cytotoxic effect on melanoma B16 cells in vitro. Lymphocytes in intact mice possess the maximum cytotoxicity for tumor cells, taken at the 20th day since the tumor inoculation, while the cytotoxic effect of mice melanoma B16 lymphocytes during this period proves to be markedly reduced. The kinetics of immune indices during the melanoma B16 growth in mice is characterized by rise and fall in the lymphocyte and sera cytotoxicity.

[In vitro humeral serum cytotoxic reactions in melanoma].

Humoral antitumor cytotoxins were found in most of assayed sera from melanoma patients. The patients' sera can cross-react in cytotoxic reactions in vitro with allogenic cell-targets of melanoma. The sera of healthy donors prove to be cytotoxic for melanoma cell-targets in vitro, the degree of their cytotoxicity being similar to that of autologous sera.

[Humoral antitumor cytotoxins in patients with melanoma of the skin].

The level of humoral antitumor cytotoxins in sera of malignant melanoma patients has been studied by means of a cytotoxic test in vitro. The authors have shown a cytotoxic effect of sera of most patients on autochthonous tumor cells of melanoma. Hela cells were used as a control cell line.