The Ubiquitin Ligase Adaptor NDFIP1 Interacts with TRESK and Negatively Regulates the Background K Current.

The TRESK (K2P18.1, KCNK18) background potassium channel is expressed in primary sensory neurons and has been reported to contribute to the regulation of pain sensations. In the present study, we examined the interaction of TRESK with NDFIP1 (Nedd4 family-interacting protein 1) in the oocyte expression system by two-electrode voltage clamp and biochemical methods.

Very high-energy electron therapy as light-particle alternative to transmission proton FLASH therapy - An evaluation of dosimetric performances.

Purpose: Clinical translation of FLASH-radiotherapy (RT) to deep-seated tumours is still a technological challenge. One proposed solution consists of using ultra-high dose rate transmission proton (TP) beams of about 200-250 MeV to irradiate the tumour with the flat entrance of the proton depth-dose profile. This work evaluates the dosimetric performance of very high-energy electron (VHEE)-based RT (50-250 MeV) as a potential alternative to TP-based RT for the clinical transfer of the FLASH effect.

Imaging phonon eigenstates and elucidating the energy storage characteristics of a honeycomb-lattice phononic crystal cavity.

We extend gigahertz time-domain imaging to a wideband investigation of the eigenstates of a phononic crystal cavity. Using omnidirectionally excited phonon wave vectors, we implement an ultrafast technique to experimentally probe the two-dimensional acoustic field inside and outside a hexagonal cavity in a honeycomb-lattice phononic crystal formed in a microscopic crystalline silicon slab, thereby revealing the confinement and mode volumes of phonon eigenstates-some of which are clearly hexapole in character-lying both inside and outside the phononic-crystal band gap. This allows us to obtain a quantitative measure of the spatial acoustic energy storage characteristics of a phononic crystal cavity.

Determining longitudinal and transverse elastic wave attenuation from zero-group-velocity Lamb waves in a pair of plates.

A method for the determination of longitudinal and transverse bulk acoustic wave attenuation from measurements of the decay-rate of two independent zero-group-velocity resonances in a couple of matched plates is presented. A linear relation is derived, which links the bulk-wave attenuation coefficients to the decay-rate of plate-resonances. The relation is used to determine the acoustic loss of tungsten at GHz frequencies from noncontact laser-ultrasonic measurements in plates with thicknesses of about 1 µm.

Critical contribution of the intracellular C-terminal region to TRESK channel activity is revealed by the epithelial Na current ratio method.

TRESK (K18.1) possesses unique structural proportions within the K background potassium channel family. The previously described TRESK regulatory mechanisms are based on the long intracellular loop between the second and the third transmembrane segments (TMS).

Translocation of TMEM175 Lysosomal Potassium Channel to the Plasma Membrane by Dynasore Compounds.

TMEM175 (transmembrane protein 175) coding sequence variants are associated with increased risk of Parkinson's disease. TMEM175 is the ubiquitous lysosomal K channel regulated by growth factor receptor signaling and direct interaction with protein kinase B (PKB/Akt). In the present study, we show that the expression of mouse TMEM175 results in very small K currents through the plasma membrane in oocytes, in good accordance with the previously reported intracellular localization of the channel.

Surface acoustic wave attenuation in polycrystals: Numerical modeling using a statistical digital twin of an actual sample.

Grain boundary scattering-induced attenuation and phase-velocity dispersion of Rayleigh-type surface acoustic waves are studied with a time-domain finite-element method (FEM). The FEM simulation incorporates a realistic material model based on matching the spatial two-point correlation function of a Laguerre tessellation with that obtained from optical micrographs of a previously studied aluminum sample. Plane surface acoustic waves are excited in a multitude of statistically equivalent virtual polycrystals, and their surface displacement fields are averaged for subsequent extraction of the coherent-wave attenuation coefficient and phase velocity.

Improvement of clinical and immunological parameters after allergen-specific immunotherapy in atopic dermatitis.

Background: Allergen immunotherapy (AIT) is considered a curative treatment in some atopic diseases, but in AD contradictory clinical results exist and the action of AIT has not been elucidated. In the literature, there is no evidence for parallel investigations of permeability barrier, cutaneous and blood immune responses after AIT in AD.

Objectives: The objective was to investigate immune parameters in the blood and skin and to detect clinical and barrier changes after AIT in AD.

Influence of grain morphology on ultrasonic wave attenuation in polycrystalline media with statistically equiaxed grains.

The influence of a polycrystals' grain structure on elastic wave scattering is studied with analytical and numerical methods in a broad frequency range. A semi-analytical attenuation model, based on an established scattering theory, is presented. This technique accurately accounts for the grain morphology without prior assumptions on grain statistics.

Optical tracking of picosecond coherent phonon pulse focusing inside a sub-micron object.

By means of an ultrafast optical technique, we track focused gigahertz coherent phonon pulses in objects down to sub-micron in size. Infrared light pulses illuminating the surface of a single metal-coated silica fibre generate longitudinal-phonon wave packets. Reflection of visible probe light pulses from the fibre surface allows the vibrational modes of the fibre to be detected, and Brillouin optical scattering of partially transmitted light pulses allows the acoustic wavefronts inside the transparent fibre to be continuously monitored.

Inverse characterization of plates using zero group velocity Lamb modes.

In the presented work, the characterization of plates using zero group velocity Lamb modes is discussed. First, analytical expressions are shown for the determination of the k-ω location of the zero group velocity Lamb modes as a function of the Poisson's ratio. The analytical expressions are solved numerically and an inverse problem is formulated to determine the unknown wave velocities in plates of known thickness.

Sebocytes differentially express and secrete adipokines.

In addition to producing sebum, sebocytes link lipid metabolism with inflammation at a cellular level and hence, greatly resemble adipocytes. However, so far no analysis was performed to identify and characterize the adipocyte-associated inflammatory proteins, the members of the adipokine family in sebocytes. Therefore, we determined the expression profile of adipokines [adiponectin, interleukin (IL) 6, resistin, leptin, serpin E1, visfatin, apelin, chemerin, retinol-binding protein 4 (RBP4) and monocyte chemoattractant protein 1 (MCP1)] in sebaceous glands of healthy and various disease-affected (acne, rosacea, melanoma and psoriasis) skin samples.

Experimental and numerical study of the excitability of zero group velocity Lamb waves by laser-ultrasound.

The excitability of zero group velocity (ZGV) Lamb waves using a pulsed laser source is investigated experimentally and through numerical simulation. Experimentally, a laser based ultrasonic technique is used to find the optical spot size on the sample surface that allows an optimal coupling of the optical energy into the ZGV mode. Numerical simulations, using the time domain finite differences technique, are carried out to model the thermoelastic generation process by laser irradiation and the propagation of the generated acoustic waves.

Comparison of numerical and effective-medium modeling of porosity in layered media.

In this study, modeling approaches for porosity in layered media are presented and compared. First, an effective-medium model is used to account for the frequency-dependent attenuation of the elastic waves. The effective-medium model is based on a single-scattering approach, i.

Characterization of the spatio-temporal response of optical fiber sensors to incident spherical waves.

In this study a theoretical framework for calculating the acoustic response of optical fiber-based ultrasound sensors is presented. The acoustic response is evaluated for optical fibers with several layers of coating assuming a harmonic point source with arbitrary position and frequency. First, the fiber is acoustically modeled by a layered cylinder on which spherical waves are impinged.

Tubulin binds to the cytoplasmic loop of TRESK background K⁺ channel in vitro.

The cytoplasmic loop between the second and third transmembrane segments is pivotal in the regulation of TRESK (TWIK-related spinal cord K+ channel, K2P18.1, KCNK18). Calcineurin binds to this region and activates the channel by dephosphorylation in response to the calcium signal.

Broadband evolution of phononic-crystal-waveguide eigenstates in real- and k-spaces.

Control of sound in phononic band-gap structures promises novel control and guiding mechanisms. Designs in photonic systems were quickly matched in phononics, and rows of defects in phononic crystals were shown to guide sound waves effectively. The vast majority of work in such phononic guiding has been in the frequency domain, because of the importance of the phononic dispersion relation in governing acoustic confinement in waveguides.

On the crossing points of the Lamb modes and the maxima and minima of displacements observed at the surface.

This article elaborates on the crossing points of the frequency-wavenumber branches for the symmetric and anti-symmetric Lamb modes in a homogeneous plate. It is shown both theoretically as well as experimentally that at these crossing points either the normal or the longitudinal components of modal displacement attain an extreme value, i.e.

Correlation among metallothionein expression, intratumoural macrophage infiltration and the risk of metastasis in human cutaneous malignant melanoma.

Background: The formation of metastases and the efficacy of systemic therapies in cutaneous malignant melanoma (CMM) depend on the characteristics of the tumour cells and the host immune response. Aberrant expression of metallothionein (MT) has been observed in several types of cancers with poor prognoses.

Objective: To perform an immunohistochemical study on primary CMM comparing the MT expression of tumours without metastases (n = 23) to that of samples with haematogenous metastases (n = 23) and to examine the correlation between MT staining and immunological markers relevant in CMM progression.

Numerical modeling of thermoelastic generation of ultrasound by laser irradiation in the coupled thermoelasticity.

Laser-generation of ultrasound is investigated in the coupled dynamical thermoelasticity in the presented paper. The coupled heat conduction and wave equations are solved using finite differences. It is shown that the application of staggered grids in combination with explicit integration of the wave equation facilitates the decoupling of the solution and enables the application of a combination of implicit and explicit numerical integration techniques.

Golay code modulation in low-power laser-ultrasound.

The current work presents a correlation-based detection technique with application in modulated laser-ultrasonics. In standard use of coded sequences the impulse response of a system is recovered in the time domain with improved signal to noise ratio (SNR). The presented method is an extension of this technique, where the response to a chirped waveform is restored with improved SNR; hence, the response is in a well-defined frequency range.

Characterization of broadband fiber optic line detectors for photoacoustic tomography.

The frequency response of fiber optic line detectors is investigated in the presented paper. An analytical model based on oblique scattering of elastic waves is used to calculate the frequency dependent acousto-optical transfer functions of bare glass optical and polymer optical fibers. From the transfer functions the transient response of fibers detectors to photoacoustically excited spherical sources is derived.

Nijmegen breakage syndrome complicated with primary cutaneous tuberculosis.

Nijmegen breakage syndrome (NBS) is a rare autosomal recessive chromosomal instability syndrome characterized by severe immunodeficiency, growth retardation, microcephaly, a distinct facial appearance, and a high predisposition to lymphoid malignancy. We report a 7-year-old white girl with NBS associated with cutaneous tuberculosis. The patient presented with multiple red-brown, centrally scaring plaques on the leg and had neither pulmonary nor systemic manifestation of tuberculosis.

Stability analysis of second- and fourth-order finite-difference modelling of wave propagation in orthotropic media.

The stability of the finite-difference approximation of elastic wave propagation in orthotropic homogeneous media in the three-dimensional case is discussed. The model applies second- and fourth-order finite-difference approaches with staggered grid and stress-free boundary conditions in the space domain and second-order finite-difference approach in the time domain. The numerical integration of the wave equation by central differences is conditionally stable and the corresponding stability criterion for the time domain discretisation has been deduced as a function of the material properties and the geometrical discretization.