Publications by authors named "Verena Oeinck"
Article Synopsis
- The study investigates the virus clearance capability of the downstream process during the manufacturing of monoclonal antibodies (mAbs), highlighting that while the protein A chromatography step is not considered a major contributor to virus removal, it still plays a role.
- By experimenting with different wash buffers and analyzing their effects on two model viruses, the Minute virus of mice (MVM) and Murine leukemia virus (xMuLV), researchers explored the interactions affecting virus retention.
- Results showed that hydrophobic interactions significantly improved virus clearance for xMuLV, while MVM retention was influenced more by hydrogen bonds and electrostatic interactions, leading to a potential for custom design wash buffers for enhanced virus removal in mAb production.
Continuous chromatography is increasingly being used across the biotechnology industry due to its economic advantages. For adoption in commercial manufacturing, also models for virus clearance studies must be available. It is demonstrated how for a virus clearance study for a multispecific antibody, the continuous protein A capture chromatography process, being run on multiple interconnected columns, can be mimicked with only a single column.
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