Is in-toing gait physiological in children? - Results of a large cohort study in 5910 healthy (pre-) school children.

Background: In-toeing is a major concern of many parents presenting their children to pediatric orthopedists. Foot progression angle (FPA) quantifies the rotation of the foot's longitudinal axis during gait, with negative values describing in-toeing and positive values describing out-toeing. Although it has been shown that the FPA changes over the course of a child's development, reference values for the normal FPA-range are lacking.

Tissue magnesium content in diabetic rats.

Diabetes mellitus is a known cause of hypomagnesemia but soft tissue magnesium depletion has not as yet been demonstrated clinically or experimentally. Direct measurements of soft tissue Mg and calcium in control, streptozotocin (STZ)-diabetic and STZ-insulin-treated-diabetic rats were made and failed to demonstrate consistent decreases in soft tissue Mg content despite the induction of modest hypomagnesemia. In addition, soft tissue Ca content was variably influenced and showed no interrelation with tissue Mg levels.

Tissue magnesium in spontaneously hypertensive rats.

Magnesium (Mg) is a vasodilator which may play a role in the regulation of blood pressure. The present study measured tissue Mg and calcium (Ca) levels in a hypertension model, the spontaneously hypertensive (SHR) rat, using the Wistar-Kyoto rat as a control. Mean serum Mg levels were normal in both types of rats.

Radiochromium distribution during saline diuresis.

Free water diuresis in vasopressin-deficient Brattleboro rats does not influence body conservation of chromium (Cr+3), suggesting a proximal tubular site for renal Cr reabsorption. Other data suggest that Cr conservation is accomplished primarily by lack of glomerular filtration or by tissue binding to a specific Cr-binding substance. To provide further data, radiochromium (51Cr) retention and tissue distribution were studied in SHR and WKY rats undergoing saline diuresis.

Radiochromium distribution in aged rats.

Fragmentary studies suggest that tissue chromium (Cr) levels decrease with age. Regardless of the mechanism for such a decline, decreased tissue exchange with administered radiochromium (51Cr) should result. Accordingly, body retention, urinary excretion, and serum (plasma) and tissue levels of 51Cr were determined in 2-month-old male control rats and in 9-18-month-old experimental male rats 3 days after the intravenous injection of high specific activity trivalent 51Cr.

Placental transport of chromium.

Since trivalent chromium (Cr+3) transport into certain tissues is rapid, the placental transport of injected high specific activity 51Cr+3 was studied in pregnant rats at days 17-20 of gestation. Three days after the intravenous injection of 51Cr+3, body retention of 51Cr was similar in pregnant and nonpregnant rats, but in the pregnant rats placentofetal uptake of 51Cr accounted for 25-30% of the 51Cr retention. The mean 51Cr content per placentofetal unit was 0.

Pancreatic exocrine function and cyclic nucleotides in the diabetic rat.

Streptozotocin-induced diabetes mellitus in the rat results in a 30% decrease in serum amylase and an 80% decrease in pancreatic amylase levels. Pancreatic trypsinogen levels decrease 50% whereas pancreatic lipase levels increase 30%. Plasma cyclic nucleotide levels (cAMP and cGMP) increase 40-100%, urine cyclic nucleotide levels decrease 75-99%, but pancreatic cyclic nucleotide levels are unchanged.

Radiochromium conservation and distribution in diuretic states.

The effect of free water diuresis by three different modalities on body 51Cr conservation and distribution was studied in adult male rats. Despite massive diuresis, ADH deficiency had little effect on body 51Cr retention or relative tissue distribution of 51Cr but did induce 25-40% increases in serum 51C concentrations which were reversible by pitressin administration. Glucose feeding produced copious diuresis but no change in body 51Cr retention or relative tissue distribution of 51Cr.

Tissue chromium exchange in the rat.

This study of chromium (Cr) exchange in the rat combined measurements of 51Cr distribution and tissue Cr content to permit an assessment of tissue Cr exchange under control conditions and during varied Cr intakes. Liver Cr was found to be 50 to 100% exchangeable whereas pancreas Cr was only 34% exchangeable. In kidney, the specific activity of 51Cr exceeded that of serum by more than 100%, indicating a complex type of exchange involving both a rapidly exchanging Cr pool and an "inner" Cr pool with "sink-like" characteristics.

Radiochromium distribution in thyroid and parathyroid deficiency.

Body retention and tissue distribution of a 51chromium (Cr) tracer were studied in thyroparathyroidectomized (TPTX) rats and in TPTX rats after replacement with thyroxin, calcitonin, or parathyroid hormone. A tracer dose containing 1 ng Cr or less and 0.5 to 0.

Radiochromium distribution in hypophysectomized and adrenalectomized rats.

The effects of hypophyseal and adrenal ablation (HYPOX, ADX) on trivalent radiochromium (51Cr) distribution and of GH and T4 replacement in HYPOX rats were determined. Hypophysectomy increased body retention of 51Cr by 20--35%, and hormonal replacement restored body retention to normal. Serum 51Cr was increased 300--800%, resulting in depressed tissue to serum 51Cr ratios.

Automated analysis of hydroxyproline with elimination of non-specific reacting substances.

A modified urinary hydroxyproline method is presented which utilizes an automated technique, Technicon instrumentation and Ehrlich's reagent. Acid hydrolysates are neutralized automatically. Non-specific reacting substances are determined simultaneously with each sample and subtracted as a blank.

An immune hemagglutination procedure with very high sensitivity applied to the detection of hepatitis B antibody and antigen and immune complex.

An immune agglutination procedure is described in which red cells are coated with antigen and agglutination is observed after a short incubation with antibody in reagent serum. Conversely, the cells may be coated with antibody and used to test for the presence of antigen. Treatment of the reagent serum with cobra venom factor results in inhibition, and there is a marked lo-s of sensitivity with cells pretreated with formalin or chromic chloride, suggesting that C3 and some type of reactive sites on the cells are required.