CRISPR-gene-engineered CYBB knock-out PLB-985 cells, a useful model to study functional impact of X-linked chronic granulomatous disease mutations: application to the G412E X91+-CGD mutation.

Chronic granulomatous disease (CGD) is a rare primary immune disorder caused by mutations in one of the five subunits of the NADPH oxidase complex expressed in phagocytes. Two-thirds of CGD cases are caused by mutations in CYBB that encodes NOX2 or gp91phox. Some rare X91+-CGD point mutations lead to a loss of function but with a normal expression of the mutated NOX2 protein.

Aliens in caves: the global dimension of biological invasions in subterranean ecosystems.

Alien species are a significant threat to natural ecosystems and human economies. Despite global efforts to address this challenge, the documented number of alien species is rapidly increasing worldwide. However, the magnitude of the impact of alien species may vary significantly across habitats.

Knowledge and perceptions of invasive plant biocontrol in Europe versus the rest of the world.

Biological control (BC) of invasive alien plants (IAP) can be an effective environmental management approach. It has only very recently been adopted in Europe and is underutilized compared to other continents where this method has been successfully implemented for over a century. This is in sharp contrast to the BC of invertebrate pests, which has been taken up widely within Europe.

The economic costs, management and regulation of biological invasions in the Nordic countries.

A collective understanding of economic impacts and in particular of monetary costs of biological invasions is lacking for the Nordic region. This paper synthesizes findings from the literature on costs of invasions in the Nordic countries together with expert elicitation. The analysis of cost data has been made possible through the InvaCost database, a globally open repository of monetary costs that allows for the use of temporal, spatial, and taxonomic descriptors facilitating a better understanding of how costs are distributed.

Making Waves: Joining forces for better doctoral education in water research.

We argue that doctoral education in water needs an elevated focus towards educating game changers who can drive innovation and change towards more sustainable futures across academic and non-academic settings. Today's doctoral graduates in water are increasingly employed outside academia, and challenged to understand complex and interconnected systems, to integrate and synthesize information from different disciplines and to lead the way toward increasingly creative, resilient, robust, and socially sustainable solutions. Supervisors are challenged by interdisciplinary research topics and by growing diversity of objectives, too often at the cost of their wellbeing.

Knowledge gaps in economic costs of invasive alien fish worldwide.

Invasive alien fishes have had pernicious ecological and economic impacts on both aquatic ecosystems and human societies. However, a comprehensive and collective assessment of their monetary costs is still lacking. In this study, we collected and reviewed reported data on the economic impacts of invasive alien fishes using InvaCost, the most comprehensive global database of invasion costs.

Validation of the Acute Physiology and Chronic Health Evaluation (APACHE) II and IV Score in COVID-19 Patients.

Background: Severity scoring systems are inherent to ICU practice for multiple purposes. Acute Physiology and Chronic Health Evaluation (APACHE) scoring systems are designed for ICU mortality prediction. This study aims to validate APACHE IV in COVID-19 patients admitted to the ICU.

Non-English languages enrich scientific knowledge: The example of economic costs of biological invasions.

We contend that the exclusive focus on the English language in scientific research might hinder effective communication between scientists and practitioners or policy makers whose mother tongue is non-English. This barrier in scientific knowledge and data transfer likely leads to significant knowledge gaps and may create biases when providing global patterns in many fields of science. To demonstrate this, we compiled data on the global economic costs of invasive alien species reported in 15 non-English languages.

10 kHz Spinal Cord Stimulation for the Treatment of Failed Back Surgery Syndrome with Predominant Leg Pain: Results from a Prospective Study in Patients from the Dutch Healthcare System.

Introduction: Persistent back/and or leg pain is a common outcome after spinal surgery (otherwise known as failed back surgery syndrome [FBSS]). Studies have shown that spinal cord stimulation (SCS) at 10 kHz provides effective analgesia in FBSS patients with both back and leg pain symptoms and in those with predominant back pain. This study is the first to evaluate the therapy in FBSS patients with predominant leg pain.

Lessons learned from rapid environmental risk assessments for prioritization of alien species using expert panels.

Limiting the spread and impacts of invasive alien species (IAS) on biodiversity and ecosystems has become a goal of global, regional and national biodiversity policies. Evidence based management of IAS requires support by risk assessments, which are often based on expert judgment. We developed a tool to prioritize potentially new IAS based on their ecological risks, socio-economic impact and feasibility of management using multidisciplinary expert panels.

Anesthesia for non-obstetric surgery during pregnancy in a tertiary referral center: a 16-year retrospective, matched case-control, cohort study.

Introduction: This retrospective, matched case-control cohort study describes the incidence, indications, anesthesia techniques and outcomes of pregnancies complicated by surgery in a single tertiary-referral hospital.

Methods: Retrospective review of the hospital records of 171 patients who had non-obstetric surgery in the current pregnancy, between 2001 and 2016. Pregnancy outcomes of these women were firstly compared with all contemporary non-exposed patients (n=35 411), and secondly with 684 non-exposed control patients, matched for age, time of delivery and parity.

Family reciprocity of older Singaporeans.

Reciprocity is a powerful motivation in social life. We study what older people give to their family for help received. Data are from the Panel on Health and Aging of Singaporean Elderly, Wave 2 (2011; persons aged 62+;  = 3103).

Pentachlorophenol, polychlorinated dibenzo-p-dioxins and polychlorinated dibenzo furans in surface soil surrounding pentachlorophenol-treated utility poles on the Kenai National Wildlife Refuge, Alaska USA.

Composite surface soil samples were collected at 0, 25, and 50 cm from the base of 12 utility poles on the Kenai National Wildlife Refuge in Alaska, to assess the extent to which pentachlorophenol, polychlorinated dibenzo-p-dioxins and polychlorinated dibenzo furans may have leached from pentachlorophenol-treated poles. Six pairs of utility poles were included, consisting of an "old" pole manufactured in 1959 or 1963, a "new" pole manufactured within the past 20 years, and a suitable background soil sample from the same vicinity. Old poles had greater concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) equivalents (TEQs) near the pole base and at 25 cm than "new" poles did.

Aging With Disability for Midlife and Older Adults.

This analysis brings "aging with disability" into middle and older ages. We study U.S.

Implementing participatory monitoring in river management: The role of stakeholders' perspectives and incentives.

Involving local stakeholders in monitoring has the potential to stimulate learning and improve management responses. However, there is limited understanding about factors which influence implementation and success. This paper reviews local stakeholders' perceptions with respect to the construction of longitudinal dams in the Dutch river Waal, and explores their incentives to be involved in monitoring the effects of this intervention.

Disability Experience and Measurement.

Top themes of international research on disability in the past three decades are discussed: disability dynamics, buffers and barriers for disability, disability trends, and disability among very old persons. Each theme is highlighted by research examples. Turning to measurement, I discuss traditional measures of disability, new longer and shorter ones, and composites like disability-free life expectancy, noting their merits.

Midlife trends in activities and disability.

Objectives: This is the first analysis that demonstrates empirically the likely tie between activities (time spent) and disability (health-related difficulty in activities). We compare trends in activities and disability for Americans ages 55 to 69 in recent years, and assess cross-sectional linkages of activities and disability.

Methods: Data are from the Health and Retirement Study, a longitudinal survey of community-dwelling U.

Exploring public perception of non-native species from a visions of nature perspective.

Not much is known about lay public perceptions of non-native species and their underlying values. Public awareness and engagement, however, are important aspects in invasive species management. In this study, we examined the relations between the lay public's visions of nature, their knowledge about non-native species, and their perceptions of non-native species and invasive species management with a survey administered in the Netherlands.

Petroleum hydrocarbon contamination in Nelson Lagoon, Alaska, sampling three different matrices.

Polycyclic aromatic hydrocarbon (PAH) levels were measured in sediments, bivalves and semi-permeable membrane devices (SPMDs) in the relatively pristine marine environment of Nelson Lagoon, Alaska. Most PAH levels in Nelson Lagoon were low, and similar to global background concentrations. Sampling media type can significantly influence conclusions of PAH contamination in the environment.

Trajectories of functional change among long stayers in nursing homes: does baseline impairment matter?

Objective: This study reports findings on functional change trajectories for long-stay residents by examining the effects of baseline medical conditions and functional status on changes in physical impairment across residents' length of stay (LOS).

Method: A 5% sample of nursing home residents from Michigan from 1999 through 2003 was used to create longitudinal episodes of care including Minimum Data Set (MDS) assessments. Data were analyzed using hierarchical linear models.

Living alone: elderly Chinese Singaporeans.

Asian societies maintain the norm that older people should live with their children. Yet some older people live alone. This is the first study to explore social isolation, strategies of coping, and preferences about living arrangements among Chinese Singaporeans aged 65+ who live alone.

Arthritis disability and heart disease disability.

Objective: Arthritis is the most common health condition in midlife and late life, and heart disease is the leading cause of death. This article compares disability impacts of these 2 preeminent health problems.

Methods: Using data from the National Health Interview Survey Disability Supplement, we studied specific limitations and disabilities, accommodations used (buffers), and accommodations needed (barriers) for US population groups of adults with arthritis disability, heart disease disability, both arthritis and heart disease disability, and disability due to other conditions.