Asymmetric flow field-flow fractionation for comprehensive characterization of hetero-aggregates made of nano-silver and extracellular polymeric substances.

The present study explores the capability of asymmetrical flow field-flow fractionation (AF4) coupled online with diode array (DAD), fluorescence detectors (FLD), multi-angle light scattering (MALS) and dynamic light scattering (DLS) to characterize silver nanoparticles (nAg) hetero-aggregates formed with diatoms derived extracellular polymeric substances (EPS). The content of EPS varied from 10.5 to 105 mgC L and nAg were dispersed at 4 mg L in a freshwater medium.

Impact of inorganic mercury on carotenoids in freshwater algae: Insights from single-cell resonance Raman spectroscopy.

The influence of inorganic mercury (Hg(II)) exposure on photosynthetic microorganisms and their pigments remains understudied. Here, we employed resonance Raman (RR) spectroscopy to investigate the responses of two freshwater phytoplankton species, the green alga Chlamydomonas reinhardtii and the diatom Cyclotella meneghiniana to Hg(II) exposure. We selectively recorded the spectral RR signature of carotenoids in intact cells exposed to concentrations of 10 nM and 100 nM of Hg(II), representative for contaminated environment and unexposed control cells.

Surface displayed MerR increases mercury accumulation by green microalga Chlamydomonas reinhardtii.

Mercury is a highly toxic trace metal that can accumulate in aquatic ecosystems and when resent at high concentrations can pose risks to both aquatic life and humans consuming contaminated fish. This research explores the use of the metalloregulatory protein MerR, known for its high affinity and selectivity toward mercury, in a novel application. Through a cell surface engineering approach, MerR was displayed on cells of green alga Chlamydomonas reinhardtii.

Exploring the impact of silver-based nanomaterial feed additives on green algae through single-cell techniques.

Silver in its various forms, including dissolved silver ions (Ag) and silver nanoparticles (AgNPs), is a promising alternative to traditional antibiotics, largely used in livestock as feed additives and could contribute to the decrease and avoidance of the development of antibiotic resistance. The present study aims to assess the potential ecotoxicity of a silver-based nanomaterial (Ag-kaolin), the feed supplemented with the nanomaterial and the faeces since the latter are the ones that finally reach the environment. To this end, green alga Raphidocellis subcapitata was exposed to the extracts of Ag-kaolin, supplemented feed, and pig faeces for 72 h, along with Ag and AgNPs as controls for comparison purposes.

Artisanal and small-scale gold mining and biodiversity: a global literature review.

Artisanal and small-scale gold mining (ASGM) is crucial to the livelihoods of close to 20 million people in over 80 countries, including 4-5 million women, mainly in rural areas with limited alternative economic prospects, particularly in developing countries. ASGM is largely informal, which can add to the challenge of addressing negative social and environmental effects including impacts on biodiversity. However, with proper guidance, ASGM can operate in a responsible manner, using cleaner production methods that minimize impacts on human health and the environment.

Exploring the subcellular distribution of mercury in green alga Chlamydomonas reinhardtii and diatom Cyclotella meneghiniana: A comparative study.

Mercury (Hg) is a priority pollutant of global concern because of its toxicity, its ability to bioaccumulate throughout the food web and reach significant concentrations in top predators. Phytoplankton bioconcentrate large amounts of Hg and play a key role in the entry of Hg into the aquatic food web. However, the subcellular distribution of Hg in freshwater phytoplankton, known to affect it toxicity and trophic transfer is understudied.

Mercury species induce metabolic reprogramming in freshwater diatom Cyclotella meneghiniana.

Mercury is a hazardous pollutant of global concern. While advances have been made in identifying the detrimental effects caused by Hg species in phytoplankton, knowledge gaps remain regarding the metabolomic perturbations induced by inorganic mercury (Hg(II)) and monomethylmercury (MeHg) in these organisms. Diatoms represent a major phytoplankton group essential in various global biogeochemical cycles.

Cytochrome c - silver nanoparticle interactions: Spectroscopy, thermodynamic and enzymatic activity studies.

Cytochrome c, an iron containing metalloprotein in the mitochondria of the cells with an oxide/redox property, plays key role in the cell apoptotic pathway. In this study, the interaction of silver nanoparticles (AgNPs) with cytochrome c (Cyt c) was investigated by using a combination of spectroscopic, imaging and thermodynamic techniques, including dynamic light scattering (DLS), ultraviolet-visible (UV-vis) spectroscopy, transmission electron microscopy (TEM), fluorescence spectroscopy, near and far circular dichroism (CD) spectroscopy, and isothermal titration calorimetry (ITC). DLS and UV-vis analysis evidenced the formation of surface complexes of Cyt c on AgNPs.

Oxidative stress induced by sub-lethal exposure to copper as a mediator in development of bacterial resistance to antibiotics.

Limited information exists on how bacterial resistance to antibiotics is acquired and altered in response to short-term metal stress, and what the prevailing pathways are. Here the precursor mechanisms of development of bacterial antibiotic resistance mediated by oxidative stress induce under sub-lethal Cu exposure were explored. The results showed that the overall level of antibiotic resistance in wild-type Escherichia coli and antibiotic-resistant E.

Metabolic alterations in alga exposed to nTiO materials.

Nano-sized titanium dioxide (nTiO) is one of the most commonly used materials, however the knowledge about the molecular basis for metabolic and physiological changes in phytoplankton is yet to be explored. In the present study we use a combination of targeted metabolomics, transcriptomics and physiological response studies to decipher the metabolic perturbation in green alga exposed for 72 h to increasing concentrations (2, 20, 100 and 200 mg L) of nTiO with primary sizes of 5, 15 and 20 nm. Results show that the exposure to all three nTiO materials induced perturbation of the metabolism of amino acids, nucleotides, fatty acids, tricarboxylic acids, antioxidants but not in the photosynthesis.

Aquatic organisms modulate the bioreactivity of engineered nanoparticles: focus on biomolecular corona.

The increased use of nanoparticle (NP)-enabled materials in everyday-life products have raised concerns about their environmental implications and safety. This motivated the extensive research in nanoecotoxicology showing the possibility that NPs could cause harm to the aquatic organisms if present at high concentrations. By contrast, studies dealing with influence that organisms could exert on the fate and thus effects of NPs are still very rare.

Comparative study of the sensitivity of two freshwater gastropods, Lymnaea stagnalis and Planorbarius corneus, to silver nanoparticles: bioaccumulation and toxicity.

Metal-based nanoparticles (NPs) are considered detrimental to aquatic organisms due to their potential accumulation. However, little is known about the mechanisms underlying these effects and their species-specificity. Here we used stable silver (Ag) NPs (20 nm, from 10 to 500 μg/L) with a low dissolution rate (≤2.

Microbial community diversity and composition in river sediments contaminated with tetrabromobisphenol A and copper.

The microbial community composition in aquatic ecosystems have received increased attention. However, the knowledge gap relative to the responses of bacterial, archaeal and fungal communities in co-contaminated river sediments remain poorly studied. Here, we investigated the changes of tetrabromobisphenol A (TBBPA) and copper (Cu) concentrations and the responses of microbial communities in co-contaminated sediments during long-term incubation.

Simple Acid Digestion Procedure for the Determination of Total Mercury in Plankton by Cold Vapor Atomic Fluorescence Spectroscopy.

Plankton, at the bottom of the food web, play a central role in the entry of mercury into the aquatic biota. To investigate their role in mercury uptake, reliable analytical procedures for Hg analysis are highly sought. Wet digestion procedures for determining total mercury in different biological matrices have been established since years, however only few studies focused on planktonic samples.

Asymmetrical Flow Field-Flow Fractionation Methods for Quantitative Determination and Size Characterization of Thiols and for Mercury Size Speciation Analysis in Organic Matter-Rich Natural Waters.

Asymmetrical flow field-flow fractionation (AF4) efficiently separates various macromolecules and nano-components of natural waters according to their hydrodynamic sizes. The online coupling of AF4 with fluorescence (Fluo) and UV absorbance (UV) detectors (FluoD and UVD, respectively) and inductively coupled plasma-mass spectrometry (ICP-MS) provides multidimensional information. This makes it a powerful tool to characterize and quantify the size distributions of organic and inorganic nano-sized components and their interaction with trace metals.

Asymmetrical Flow Field-Flow Fractionation Coupled to ICP-MS for Characterization of Trace Metal Species in the Environment from Macromolecular to Nano-Assemblage Forms: Current Challenges for Quantification.

Asymmetrical flow field-flow fractionation (AF4) is a powerful technique employed for the separation of macromolecules, nanoparticles, and their assemblages according to their hydrodynamic behavior. It is well known that at this size range, complex interactions can occur between components (e.g.

Dual role of titanium dioxide nanoparticles in the accumulation of inorganic and methyl mercury by crustacean Daphnia magna through waterborne and dietary exposure.

Titanium dioxide nanoparticles (nTiO) are widely used in numerous products, yet their role in the accumulation and transfer of other contaminants in the aquatic food webs is not well understood. The influence of nTiO on inorganic (IHg) and monomethyl mercury (MeHg) accumulation in invertebrate Daphnia magna through waterborne and dietary exposure was thus thoroughly investigated. The results showed that nTiO led to a substantial decrease of the total mercury body burden (THg) in D.

Determination of the Intracellular Complexation of Inorganic and Methylmercury in Cyanobacterium sp. PCC 6803.

Understanding of mercury (Hg) complexation with low molecular weight (LMW) bioligands will help elucidate its speciation. In natural waters, the rate of this complexation is governed by physicochemical, geochemical, and biochemical parameters. However, the role of bioligands involved in Hg intracellular handling by aquatic microorganisms is not well documented.

Light-trapped caddisflies to decipher the role of species traits and habitats in Hg accumulation and transfer.

We present a novel meta-community approach to explore the influence of species traits, such as adult body size, larval feeding type and microhabitat, as well as larval macrohabitat (main river channel vs. floodplain water bodies) on the concentration of total Hg accumulated ([THg]) in assemblages of adult caddisflies. We analyzed [THg] in 157 light-trapped adult caddisflies in a floodplain sector of the French upper Rhône River and used a linear mixed effect model to decipher the role of species traits and habitats in Hg accumulation.

Species-specific isotope tracking of mercury uptake and transformations by pico-nanoplankton in an eutrophic lake.

The present study aims to explore the bioaccumulation and biotic transformations of inorganic (iHg) and monomethyl mercury (MMHg) by natural pico-nanoplankton community from eutrophic lake Soppen, Switzerland. Pico-nanoplankton encompass mainly bacterioplankton, mycoplankton and phytoplankton groups with size between 0.2 and 20 μm.

Metabolomic Responses of Green Alga Exposed to Sublethal Concentrations of Inorganic and Methylmercury.

Metabolomics characterizes low-molecular-weight molecules involved in different biochemical reactions and provides an integrated assessment of the physiological state of an organism. By using liquid chromatography-mass spectrometry targeted metabolomics, we examined the response of green alga to sublethal concentrations of inorganic mercury (IHg) and monomethylmercury (MeHg). We quantified the changes in the levels of 93 metabolites preselected based on the disturbed metabolic pathways obtained in a previous transcriptomics study.

Distinguishing the effects of Ce nanoparticles from their dissolution products: identification of transcriptomic biomarkers that are specific for ionic Ce in Chlamydomonas reinhardtii.

Cerium (Ce) is a rare earth element that is incorporated in numerous consumer products, either in its cationic form or as engineered nanoparticles (ENPs). Given the propensity of small oxide particles to dissolve, it is unclear whether biological responses induced by ENPs will be due to the nanoparticles themselves or rather due to their dissolution. This study provides the foundation for the development of transcriptomic biomarkers that are specific for ionic Ce in the freshwater alga, Chlamydomonas reinhardtii, exposed either to ionic Ce or to two different types of small Ce ENPs (uncoated, ∼10 nm, or citrate-coated, ∼4 nm).

Morphological plasticity in Chlamydomonas reinhardtii and acclimation to micropollutant stress.

Phytoplankton are characterized by a great phenotypic plasticity and amazing morphological variability, both playing a primary role in the acclimation to changing environments. However, there is a knowledge gap concerning the role of algal morphological plasticity in stress responses and acclimation to micropollutants. The present study aims at examining palmelloid colony formation of the green alga Chlamydomonas reinhardtii upon micropollutants exposure.