Development and validation of HPLC method with fluorometric detection for quantification of bisnaphthalimidopropyldiaminooctane in animal tissues following administration in polymeric nanoparticles.

A simple, sensitive and specific high-performance liquid chromatography method for the quantification of bisnaphthalimidopropyldiaminooctane (BNIPDaoct), a potent anti-Leishmania compound, incorporated into poly(d,l-lactide-co-glycolic acid) (PLGA) nanoparticles was developed and validated toward bioanalysis application. Biological tissue extracts were injected into a reversed-phase monolithic column coupled to a fluorimetric detector (λexc=234nm, λem=394nm), using isocratic elution with aqueous buffer (acetic acid/acetate 0.10M, pH 4.

Evaluation of acetylcysteine promoting effect on CdTe nanocrystals photoluminescence by using a multipumping flow system.

A simple and straightforward quantification method integrated in a fully automated multi-pumping flow system (MPFS) using water-soluble mercaptopropionic acid (MPA)-capped CdTe quantum dots (QDs) was implemented for the fluorescence quantification of N-acetyl-L-cysteine (NAC) in pharmaceutical formulations. The developed approach was based on NAC ability to establish surface interactions that result in enhanced nanocrystals fluorescence intensity, proportional to analyte concentration. Size and concentration of QDs, ageing, composition, concentration and pH of the buffer solution revealed to have a noticeable effect on the enhancing efficiency affecting sensitivity and linear working range of the methodology.

Application of quantum dots as analytical tools in automated chemical analysis: a review.

Colloidal semiconductor nanocrystals or quantum dots (QDs) are one of the most relevant developments in the fast-growing world of nanotechnology. Initially proposed as luminescent biological labels, they are finding new important fields of application in analytical chemistry, where their photoluminescent properties have been exploited in environmental monitoring, pharmaceutical and clinical analysis and food quality control. Despite the enormous variety of applications that have been developed, the automation of QDs-based analytical methodologies by resorting to automation tools such as continuous flow analysis and related techniques, which would allow to take advantage of particular features of the nanocrystals such as the versatile surface chemistry and ligand binding ability, the aptitude to generate reactive species, the possibility of encapsulation in different materials while retaining native luminescence providing the means for the implementation of renewable chemosensors or even the utilisation of more drastic and even stability impairing reaction conditions, is hitherto very limited.