- Mediated transformation of (L.) Kurz.

In the present study, -mediated transformation has been optimized for (L.) Kurz. Precultured axenic leaf segments were co-cultivated with strain LBA4404 harboring the binary plasmid pCAMBIA1301 with β-glucuronidase () containing intron as the reporter gene and hygromycin phosphotransferase () as a selectable marker gene.

Subcellular localization of proteins of Oryza sativa L. in the model tobacco and tomato plants.

The cellular localization and molecular interactions are indicative of functions of a protein. The development of a simple and efficient method for subcellular localization of a protein is indispensable to elucidate gene function in plants. In this study, we assessed the feasibility of Agrobacterium-mediated transformation (agroinfiltration) of tobacco and tomato leaf tissue to follow intracellular targeting of proteins from rice fused to green fluorescent protein (GFP).