Retting and degumming of natural fibers by pectinolytic enzymes produced from Bacillus tequilensis SV11-UV37 using solid state fermentation.

The present study demonstrated the simultaneous production and optimization of pectinolytic enzymes (pectate lyase and polygalacturonase) under SSF from Bacillus tequilensis SV11-UV37 using wheat bran as a substrate, which is commercially viable and cost-effective. Optimization by one variable-at-a-time-approach showed a maximum yield of pectate lyase (1371.25 U/gds) and polygalacturonase (85.

Bioconversion of lignocellulosic biomass to xylitol: An overview.

Lignocellulosic wastes include agricultural and forest residues which are most promising alternative energy sources and serve as potential low cost raw materials that can be exploited to produce xylitol. The strong physical and chemical construction of lignocelluloses is a major constraint for the recovery of xylose. The large scale production of xylitol is attained by nickel catalyzed chemical process that is based on xylose hydrogenation, that requires purified xylose as raw substrate and the process requires high temperature and pressure that remains to be cost intensive and energy consuming.

Streptosporangium terrae sp. nov., a novel actinomycete isolated from the rhizosphere of Callistemon citrinus (Curtis), India.

A novel actinomycete strain, designated VRC21(T), was isolated from the rhizosphere of Callistemon citrinus collected from Hyderabad, India. The morphological and chemotaxonomic properties of strain VRC21(T) was consistent with the characteristics of members of the genus Streptosporangium, that is, the formation of sporangia on aerial mycelium, coiled unbranched hyphae within the spore vesicle, the presence of meso-diaminopimelic acid in the cell wall, and madurose and galactose as major whole-cell sugars. Diagnostic polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol-mannosides.

A novel thermostable, alkaline pectate lyase from Bacillus tequilensis SV11 with potential in textile industry.

An extracellular pectate lyase was purified and characterized from a UV mutant of Bacillus tequilensis SV11. Purification resulted in a 16.2-fold improvement in the enzyme specific activity, with approximately 40.

Saccharopolyspora indica sp. nov., an actinomycete isolated from the rhizosphere of Callistemon citrinus (Curtis).

A novel actinomycete strain, designated VRC122T, was isolated from a Callistemon citrinus rhizosphere sample collected from New Delhi, India, and its taxonomic status was determined by using a polyphasic approach. Strain VRC122T was a Gram-stain-positive, aerobic, non-motile, non-acid-alcohol-fast strain. Phylogenetic analysis based on 16S rRNA gene sequences showed the strain was placed in a well-separated sub-branch within the genus Saccharopolyspora.

Nocardia bhagyanesis sp. nov., a novel actinomycete isolated from the rhizosphere of Callistemon citrinus (Curtis), India.

A novel actinomycete strain, designated VRC07(T), was isolated from a Callistemon citrinus rhizosphere sample collected from Hyderabad, India. Its taxonomic status was determined by using polyphasic approach. It is a Gram-positive, aerobic, non-motile, weakly acid-fast strain.

Evaluation of pretreatment methods for enzymatic saccharification of wheat straw for bioethanol production.

Pretreatment is an essential step in the enzymatic hydrolysis of biomass and subsequent production of bioethanol. The current study is focused on two different pretreatment methods of wheat straw using mild temperatures (100°C for 2h and RT for overnight). In one method, native substrate was treated with 1.

Effect of chemical and microbial vitamin B₁₂ analogues on production of vitamin B₁₂.

Strain improvement by genetic manipulation or optimization of fermentation conditions for overproduction of vitamin B(12) has a drawback due to feed back inhibition. To resist the feed back inhibition by analogues of vitamin B(12) in Propionibacterium freudenrechii subsps. shermanii (OLP-5), we have tested with microbially separated B(12) analogues from three different strains.