Selective breast cancer cell capture, culture, and immunocytochemical analysis using self-assembled magnetic bead patterns in a microfluidic chip.

Separation and subsequent culturing of MCF-7 breast cancer cells on self-assembled protein-coated magnetic beads in a microfluidic chip is demonstrated. The beads were patterned in situ inside a sealed microfluidic channel using magnetic-field-assisted electrostatic self-assembly. Hereafter, they were grafted by exposure to a solution of 5D10 monoclonal antibodies (mAb) and fibronectin (FN), with the first being used for immunospecific cell capture and the latter being used for cell adhesion and growth.

Bioconjugated lanthanide luminescent helicates as multilabels for lab-on-a-chip detection of cancer biomarkers.

The lanthanide binuclear helicate [Eu(2)(L(C2(CO(2)H)))(3)] is coupled to avidin to yield a luminescent bioconjugate EuB1 (Q = 9.3%, tau((5)D(0)) = 2.17 ms).

Time-resolved lanthanide luminescence for lab-on-a-chip detection of biomarkers on cancerous tissues.

PDMS-based microfluidic devices combined with lanthanide-based immunocomplexes have been successfully tested for the multiplex detection of biomarkers on cancerous tissues, revealing an enhanced sensitivity compared to classical organic dyes.

On-chip immunoassay using electrostatic assembly of streptavidin-coated bead micropatterns.

We propose an original concept for a sandwich immunoassay that is completely performed on-chip using streptavidin-coated beads as substrate. The latter are electrostatically self-assembled on aminosilane micropatterns at the bottom of a microfluidic channel. We use mouse IgG diluted in phosphate buffered saline (PBS) with 1% bovine serum albumin (BSA) solution as target antigen.