Paternal exclusion: allele sharing in microsatellite testing.

Background: A paternity disagreement analyzed with 15 autosomal microsatellite markers indicated allele sharing between the mother, questioned child and the alleged father generating an inconclusive paternity result.

Design And Methods: In total, 15 autosomal and 17 Y tandem repeat loci were analyzed using AmpF/STR Identifiler, AmpF/STR Y-filer kits followed by six microsatellite markers on X chromosome in DNA extracted from peripheral blood samples of the mother, questioned child and alleged father.

Results: Microsatellite analysis examined with 15 autosomal short tandem repeats (STRs) indicated at least one allele sharing at 14 loci between the mother, questioned child and alleged father except for the TPOX locus where a paternal-child allele mismatch was observed.

Single and double incompatibility at vWA and D8S1179/D21S11 loci between mother and child: implications in kinship analysis.

Background: Two cases of paternity dispute, examined with 17 autosomal short tandem repeats signified a possible single and double maternal mismatch at vWA and D8S1179/D21S11 loci in the children under investigation.

Methods: Seventeen autosomal STR loci were analyzed using AmpFlSTR Identifiler, PowerPlex 16 kits. Six STR markers on X chromosome were amplified and analyzed.

Mother-child double incompatibility at vWA and D5S818 loci in paternity testing.

Background: In a paternity dispute case, 17 autosomal short tandem repeats (STR) were examined and signified a possible paternal mismatch at vWA locus and a maternal mismatch at D5S818 locus in the child under investigation.

Methods: Seventeen autosomal, 17 Y-chromosome and six X-chromosome repeat loci were used in parentage analysis. The mutated vWA and D5S818 alleles were amplified, cloned and sequenced to analyze the repeat structure.

Microsatellite mutation in the maternally/paternally transmitted D18S51 locus: two cases of allele mismatch in the child.

Background: We investigated 2 cases of paternity dispute with 17 autosomal short tandem repeats (STR), that indicated a mismatch to the maternally and paternally inherited allele at D18S51 locus in children under inquiry.

Methods: 17 autosomal and Y STR loci were analyzed using AmpFlSTR Identifiler, PowerPlex 16, AmpFlSTR(R)Y-filertrade mark kits. The mitochondrial DNA hypervariable regions HV1 and HV2 and 6 STR markers on X chromosome were amplified and sequenced.