Analysis of the effect of concentration and magnetic field on bioactivity of humic acids from position of open non-equilibrium systems.

On the example of cultures of bacteria Pseudomonas sp. and Bacillus sp. the effect of humic acids in the concentration range (0-15 mg/L) on the viability of these bacteria was studied.

[THE INFLUENCE OF B-IRRADIATION ON MICROMYCETES ISOLATED FROM THE ZONE OF RADIONUCLIDE CONTAMINATION].

The resistance of the micromycetes to β-irradiation had been studied. The study was conducted on two species: Cladosporium sphaerospermum Penz. and Alternaria alternata (Fr.

[Veterinary probiotic preparation influence on the biochemical blood indices of young farm animals].

The veterinary probiotic preparation was developed on the basis of aerobic part of bacterial strains of normal human intestinal microflora: 3 strains of Escherichia coli--G35No1-413, G35No2-412, G35No3-411, and one Enterococcus faecalis G35No4-410 strain. The influence of a pilot lot of the prepapration on the biochemical blood indices and the state of intestinal microflora of different age groups of young farm animal (calves and piglets) was studied during the process of the commission tests. It has been shown, that the developed probiotic promotes the improvement of protein and mineral metabolism, normalization of level of enzyme activities, which show state of the native immunity of the young farm animals' organism.

[Effect of metal microelements on biochemical indices of probiotic bacteria].

The influence of metal-microelements (Zn, Cu, Ag, Au) in colloid and ionic form on the main biochemical parameters (ATP-ase activity, transmembrane potential and respiratory activity) of E. coli G35 N#1-413 and Ent. faecalis G35 N#4-410 probiont-strains has been studied with the goal to create complex metal-bearing probiotic preparations.

[Obtaining of bacterial bioluminescent strain Protobacterium phosphoreum B7071 (lux+) for the determination of zinc ion concentration].

The transconjugate containing hybrid plasmid (Te(R)Zn(R)lux+) was obtained using the conjugation method on Pseudomonasfragi T2(5) (Te(R)ZnR) strain and bioluminescent strain Protobacterium phosphoreum B7071 (lux+). The expression regulation of lux-genes on the obtained plasmid is carried out by promotor-operational area conjointly with zinc resistance genes. The cells of the obtained genetically modified bacteria have the ability to specific induced luminescence, which is a respond to zinc ions' presence in the measuring medium.

[Structural and functional aspects of bacterial membranes and liposomes interactions].

Effect of exogenous lipids on the morphology and enzymatic activity of Bacillus cereus B4368 membrane has been studied. Specific character of different lipids action on the membrane enzyme activity was found. This peculiarity had been taken into account by using liposomes in biological systems.

[Obtaining bioluminescent bacterial strains for detection of thallium ions].

The possibility of Alcaligenes eutrophus T1 luxphenotype creation by temperature induced mutagenesis has been shown. These mutants are able to emit light after induction by thallium ions. This biological method of thallium detection possesses high specificity and sensitivity (0.

[Effect of colloid gold on physiologic-biochemical processes in Escherichia coli 1257].

The influence of colloid gold on the growth processes, ATP-ase activity and extrusion of protons in Escherichia coli 1257 was studied. The particles of colloid gold exert nonmonotonous influence on these processes with different direction is such a way that small concentration of this metal (5 x 10(-7)-5 x 10(-6) mg/ml) exert stimulative effect, while higher concentrations of colloid gold result in the suppression of biological activity of the bacterial cells. The discovered peculiarities of colloid gold influence of E.

[Comparative analysis of radial linear growth velocity of micromycetes isolated from different ecotopes].

The work aim was the discovering of peculiarities of microscopic fungi responding to radioactive pollution by growth velocity change. The linear growth velocity of a broad range of micromycetes was analyzed. They were chosen in such a way that every species, as far as possible, was presented by the strains isolated from radioactive polluted and clean regions.

[Peculiarities of linear growth of the melanin-containing fungi Cladosporium sphaerospermum Penz. and Alternaria alternata (Fr.) Keissler].

Results on determination of radial (linear) growth rate of melanin-containing fungi Cladosporium sphaerospermum Penz. and Alternaria alternata (Fr.) Keissler concerning place of their isolation and ecologic peculiarities are presented in the paper.

[The effect of gamma irradiation on the physiological-biochemical properties of strains of Cladosporium cladosporioides (Fres.) de Vries differing by the trait of radiotropism].

The influence of the low-intensity gamma-irradiation on the process of lipid peroxidation and the activities of the antioxidant glutathione-dependent system (catalase, glutathione-transferase) has been investigated in a number of Cladosporium cladosporioides (Fres.) de Vries strains. The dark-pigmented strains isolated from the habitats with different degree of radionuclide contamination, and the nonpigmented alb-mutant of the same species have been used in our work.

[The protein-synthesizing activity of a number of strains of Cladosporium cladosporioides (Fres.) de Vries grown under conditions of continuous low-intensity gamma irradiation].

Phenomena of melanization of radioactively polluted soils, due to prevalence of melanin-containing species and positive radiotropism of some micromycetes have been found during monitoring of mycobiota of the 30-km alienation zone of the Chernobyl NPP for 10 years. To elucidate the contribution of the melanin system to the cell protection against irradiation, the influence of gamma-irradiation on the activity of protein synthesis in four Cladosporium cladosporioides (Fres.) de Vries strains has been investigated.