Efficacy of chitosan root canal medicament against cross-kingdom dual-species biofilm of Candida albicans and Enterococcus faecalis in an in vitro root-canal model.

Infection of the root canals with cross-kingdom biofilm of Candida albicans (C. albicans) and Enterococcus faecalis (E. faecalis) causes more inflammation and is resistant to conventional medication.

Effects of lipopolysaccharide on proliferation, migration and osteogenic differentiation of apical papilla cells from early and late stage of root development.

The aim of this study was to investigate the effects of lipopolysaccharide on cell proliferation, migration and osteogenic differentiation of apical papilla cells from early and late stage of root development. After challenging with various lipopolysaccharide concentrations to apical papilla cells from both stages of root development for 168 h, cell proliferation and migration were investigated. Osteogenic differentiation was examined by Alizarin red staining, and gene expressions of bone/cementum or dentin-related genes were examined by polymerase chain reaction.

Mechanical stress induced S100A7 expression in human dental pulp cells to augment osteoclast differentiation.

Objectives: Mechanical injury of dental pulp leads to root resorption by osteoclasts/odontoclasts. S100 proteins have been demonstrated to be involved in inflammatory processes and bone remodeling. This study aimed to investigate the effect of mechanical stress on S100A7 expression by human dental pulp cells (HDPCs) and the effect of S100A7 proteins on osteoclast differentiation.

Effects of Lipopolysaccharide on the Proliferation and Osteogenic Differentiation of Stem Cells from the Apical Papilla.

Introduction: Stem cells from the apical papilla (SCAPs) were suggested as the stem cell source in regenerative endodontic procedures. However, bone and/or cementum-like structure were observed in root canals. Lipopolysaccharide (LPS) in infected root canals might alter SCAPs' osteogenic differentiation pattern.

Comparison of stem cell behaviors between indigenous high and low-CD24 percentage expressing cells of stem cells from apical papilla (SCAPs).

CD24 was suggested as a marker to SCAPs and has been reported for a decade. CD24 has been shown to involve stem cell activities such as self-renewal, proliferation and differentiation. However, the percentage variations of CD24 positive cells were reported among the studies.

Response of inflamed pulps of rat molars after capping with pulp-capping material containing fluocinolone acetonide.

Introduction: Capping inflamed dental pulp tissue is currently a controversial issue. To reduce pulp inflammation and stimulate pulp healing, a pulp-capping material containing fluocinolone acetonide (PCFA) has been developed. This study was aimed to evaluate the inflammatory response and hard tissue formation of inflamed dental pulps of rat maxillary molars after capping with Dycal, mineral trioxide aggregate (MTA), or PCFA.

Incorporation of anti-inflammatory agent into calcium hydroxide pulp capping material: an in vitro study of physical and mechanical properties.

The aims of this study were to investigate the release of fluocinolone acetonide from an experimental pulp capping material containing fluocinolone acetonide (PCFA) and compare some physical and mechanical properties with Dycal(®). The PCFA is a hard-setting calcium hydroxide cement composed of 50 mmol/L fluocinolone acetonide. Conditioned media from the setting material was collected for determination of fluocinolone acetonide release by high performance liquid chromatography and pH measurement by pH meter.

Setting time and flowability of accelerated Portland cement mixed with polycarboxylate superplasticizer.

Introduction: Important limitations of mineral trioxide aggregate for use in clinical procedures are extended setting time and difficult handling characteristics. The removal of gypsum at the end stage of the Portland cement manufacturing process and polycarboxylate superplasticizer admixture may solve these limitations.

Methods: Different concentrations of polycarboxylate superplasticizer (0%, 1.

Effect of fluocinolone acetonide on human dental pulp cells: cytotoxicity, proliferation, and extracellular matrix formation.

Introduction: The goal of vital pulp therapy is to maintain pulp vitality and function. Fluocinolone acetonide is a potent topical glucocorticoid used in the treatment of skin disorders and oral lesions that could possibly be used to resolve inflammation and stimulate the healing process of inflamed dental pulp. The purpose of this study was to investigate the effects of fluocinolone acetonide (0.

Stress distribution with different restorations in teeth with curved roots: a finite element analysis study.

Introduction: The aim of this study was to investigate and compare stress distribution in various degrees of curved roots with different post and restorations by finite element analysis (FEA).

Methods: Sixteen 3-dimensional FEA models were created and adapted by using computer software based on external anatomy of mandibular second premolar. A 50-N load was applied.

Finite element analysis and strain-gauge studies of vertical root fracture.

Vertical root fracture seems to result from stresses generated within the root canal and typically occurs in a buccolingual direction through the thickest part of dentin. Because stresses in the canal wall are difficult to measure experimentally, we have attempted to correlate stress patterns derived from finite element models of maxillary and mandibular incisors with strain measurements on the root surfaces of extracted teeth. Finite element analysis indicated that circumferential tensile stresses were concentrated on the buccal and lingual surfaces of the canal wall, corresponding to areas of greatest canal wall curvature.

Patterns of vertical root fracture: factors affecting stress distribution in the root canal.

Previous studies have indicated that vertical root fracture tends to occur in a buccolingual direction, where dentin thickness is greatest. Factors potentially influencing the location and direction of root fracture include root canal shape, external root morphology, and dentin thickness. In this finite-element study, simulated root sections were varied systematically with respect to canal size and shape, external root morphology, and dentin thickness to determine their relative contribution to vertical root fracture.

Effects of root canal sealers on vertical root fracture resistance of endodontically treated teeth.

The aim of this study was to compare vertical forces at fracture of endodontically treated mandibular incisors obturated with different types of root canal sealer. Four groups of 10 teeth each were tested; group 1 served as positive and negative controls (five teeth each), and groups 2, 3, and 4 were obturated by lateral condensation with gutta-percha and AH Plus, Tubliseal, or Ketac-Endo, respectively. All teeth were loaded vertically using a plugger tip inserted into the canal space until fracture occurred; the load at fracture and the pattern of fracture were recorded.