Serialized on-grid lift-in sectioning for tomography (SOLIST) enables a biopsy at the nanoscale.

Cryo-focused ion beam milling has substantially advanced our understanding of molecular processes by opening windows into cells. However, applying this technique to complex samples, such as tissues, has presented considerable technical challenges. Here we introduce an innovative adaptation of the cryo-lift-out technique, serialized on-grid lift-in sectioning for tomography (SOLIST), addressing these limitations.

Lipid Droplets Define a Sub-Population of Breast Cancer Stem Cells.

Tumor recurrence is now the leading cause of breast cancer-related death. These recurrences are believed to arise from residual cancer stem cells that survive initial therapeutic intervention. Therefore, a comprehensive understanding of cancer stem cell biology is needed to generate more effective therapies.

The relationship between plasma carotenoids and depressive symptoms in older persons.

Objective: We examined the cross-sectional and longitudinal relationship between plasma carotenoids and depressive symptoms over a 6-year follow-up in older persons.

Methods: This research is part of the InCHIANTI Study, a prospective population-based study of older persons in Tuscany, Italy. The sample for this analysis included 958 women and men aged 65 years and older.

Frailty and the homeostatic network.

Trajectories of health and functioning with age show extreme variability among different individuals. In frail, older persons the decline in functional reserve is accelerated and compensatory mechanisms start failing, with high risk of homeostasis disruption and consequent negative health outcomes. Frailty is currently conceptualized as an age-related alteration in physiology and pathology that results into a typical constellation of signs and symptoms.

Serum 25-hydroxyvitamin D and depressive symptoms in older women and men.

Context: Hypovitaminosis D and depressive symptoms are common conditions in older adults.

Objective: We examined the relationship between 25-hydroxyvitamin D [25(OH)D] and depressive symptoms over a 6-yr follow-up in a sample of older adults.

Design And Setting: This research is part of a population-based cohort study (InCHIANTI Study) in Tuscany, Italy.

Trail Making Test predicts physical impairment and mortality in older persons.

Objectives: To examine whether performance in the Trail Making Test (TMT) predicts mobility impairment and mortality in older persons.

Design: Prospective cohort study.

Setting: Community-dwelling older persons enrolled in the Invecchiare in Chianti (InCHIANTI) Study.

Personal mastery and lower body mobility in community-dwelling older persons: the Invecchiare in Chianti study.

Objectives: To test the hypothesis that, in older persons, sense of personal mastery, defined as the extent to which one regards one's life chance as being under one's own control, predicts change in lower extremity performance during a 6-year follow-up.

Design: Prospective cohort study.

Setting: Community based.