Rapid In Situ Near-Infrared Assessment of Tetrahydrocannabinolic Acid in Cannabis Inflorescences before Harvest Using Machine Learning.

Cannabis is cultivated for therapeutic and recreational purposes where delta-9 tetrahydrocannabinol (THC) is a main target for its therapeutic effects. As the global cannabis industry and research into cannabinoids expands, more efficient and cost-effective analysis methods for determining cannabinoid concentrations will be beneficial to increase efficiencies and maximize productivity. The utilization of machine learning tools to develop near-infrared (NIR) spectroscopy-based prediction models, which have been validated from accurate and sensitive chemical analysis, such as gas chromatography (GC) or liquid chromatography mass spectroscopy (LCMS), is essential.

Developing Prediction Models Using Near-Infrared Spectroscopy to Quantify Cannabinoid Content in .

Cannabis is commercially cultivated for both therapeutic and recreational purposes in a growing number of jurisdictions. The main cannabinoids of interest are cannabidiol (CBD) and delta-9 tetrahydrocannabidiol (THC), which have applications in different therapeutic treatments. The rapid, nondestructive determination of cannabinoid levels has been achieved using near-infrared (NIR) spectroscopy coupled to high-quality compound reference data provided by liquid chromatography.

Quantitation and Distribution of -Derived Alkaloids in Perennial Ryegrass Tissues.

Perennial ryegrass ( L.), an economically important pasture and turf grass, is commonly infected with asexual species endophytes. Endophytes provide enhanced bioprotection by producing alkaloids, and research often focusses on the negative impact on grazing animals.

The Known Antimammalian and Insecticidal Alkaloids Are Not Responsible for the Antifungal Activity of Endophytes.

Asexual sp. endophytes in association with pasture grasses produce agronomically important alkaloids (e.g.

Ergot alkaloid mycotoxins: physiological effects, metabolism and distribution of the residual toxin in mice.

The complex ergot alkaloids, ergovaline and ergotamine, cause dysregulation of physiological functions, characterised by vasoconstriction as well as thermoregulatory and cardiovascular effects in grazing livestock. To assess the effect of the mycotoxins, blood pressure and heart rate of male mice were measured, and metabolite profiling undertaken to determine relative abundances of both ergotamine and its metabolic products in body and brain tissue. Ergotamine showed similar cardiovascular effects to ergovaline, causing elevations in blood pressure and reduced heart rate.

Identification and Distribution of Novel Metabolites of Lolitrem B in Mice by High-Resolution Mass Spectrometry.

Lolitrem B is the most potent indole-diterpene mycotoxin produced by var. (termed TG-1), with severe intoxication cases reported in livestock. To date, there are no in vivo metabolism studies conducted for the mycotoxin.

Analysis of the Indole Diterpene Gene Cluster for Biosynthesis of the Epoxy-Janthitrems in Endophytes.

Epoxy-janthitrems are a class of indole diterpenes with structural similarity to lolitrem B. Two taxa of asexual endophytes have been reported to produce epoxy-janthitrems, TG-3 ( Taxonomic Group 3; e.g.

A Simple LC-MS Method for the Quantitation of Alkaloids in Endophyte-Infected Perennial Ryegrass.

The rapid identification and quantitation of alkaloids produced by endophyte-infected pasture grass is important for the agricultural industry. Beneficial alkaloids, such as peramine, provide the grass with enhanced insect protection. Conversely, ergovaline and lolitrem B can negatively impact livestock.

NMR Based Metabolomic Analysis of Health Promoting Phytochemicals in Lentils.

Lentils are a high-protein plant food and a valuable source of human nutrition, particularly in the Indian subcontinent. However, beyond sustenance, there is evidence that the consumption of lentils (and legumes in general) is associated with decreased risk of diseases, such as diabetes and cardiovascular disease. Lentils contain health-promoting phytochemicals, such as trigonelline and various polyphenolics.

Tremorgenic Mycotoxins: Structure Diversity and Biological Activity.

Indole-diterpenes are an important class of chemical compounds which can be unique to different fungal species. The highly complex lolitrem compounds are confined to species, whilst penitrem production is confined to spp. and spp.

Elevated CO and virus infection impacts wheat and aphid metabolism.

Introduction: The aphid Rhopalosiphum padi L. is a vector of Barley yellow dwarf virus (BYDV) in wheat and other economically important cereal crops. Increased atmospheric CO has been shown to alter plant growth and metabolism, enhancing BYDV disease in wheat.

Thermoelectric Heat Patch for Clinical and Self-Management: Melanoma Excision Wound Care.

Thermotherapy is considered to have potential beneficial effects when applied to wounds. Of particular relevance to this research are wounds that have dropped in temperature due to regional anaesthesia. This study is aimed at developing a normothermic system comprising of a heat patch controlled by external hardware.

Insect-plant-pathogen interactions as shaped by future climate: effects on biology, distribution, and implications for agriculture.

Carbon dioxide (CO ) is the main anthropogenic gas which has drastically increased since the industrial revolution, and current concentrations are projected to double by the end of this century. As a consequence, elevated CO is expected to alter the earths' climate, increase global temperatures and change weather patterns. This is likely to have both direct and indirect impacts on plants, insect pests, plant pathogens and their distribution, and is therefore problematic for the security of future food production.

The effect of elevated CO and virus infection on the primary metabolism of wheat.

Atmospheric CO2 concentrations are predicted to double by the end of this century. Although the effects of CO2 fertilisation in crop systems have been well studied, little is known about the specific interactions among plants, pests and pathogens under a changing climate. This growth chamber study focuses on the interactions among Barley yellow dwarf virus (BYDV), its aphid vector (Rhopalosiphum padi) and wheat (Triticum aestivum L.

The effect of elevated temperature on Barley yellow dwarf virus-PAV in wheat.

Barley yellow dwarf virus-PAV (BYDV-PAV) is associated with yellow dwarf disease, one of the most economically important diseases of cereals worldwide. In this study, the impact of current and future predicted temperatures for the Wimmera wheat growing district in Victoria, Australia on the titre of BYDV-PAV in wheat was investigated. Ten-day old wheat (Triticum aestivum, cv.

Sedimentation behaviour in electrorheological fluids based on suspensions of zeolite particles in silicone oil.

Sedimentation is a known and expected shortcoming of electrorheological fluids (ERFs) due to the inherent difference in the constituent densities. The long-term sedimentation causes loss of the electrorheological phenomenon and the exploitable electromechanical and viscoelastic properties despite the presence of the stimulating electric field. In this work, we report the effect of temperature and surfactant concentration on the stability of ERFs prepared from zeolite particles and silicone oil with primary focus on the sedimentation of the particles in the ERF.

De materia medica versus codex alimentarius for the reinforcement of the gynecologic immune system: the case of endometriosis.

Since the first Pharmacopoeia under the title "De Materia Medica," the importance of the utilization of plants and herbs has been an invaluable medicinal tool successfully employed for strengthening the immune system for combating a number of diseases in general, or assisting fertility and reproductive issues in particular. The beneficial use of herbal extracts, constituting the basis of modern medicines, is lately under the shadow of Codex Alimentarius that threatens, if not properly applied, serious immunity features rendering the host defenseless for intercepting harmful invaders, one of which is the mesenchymal endometriotic stem cell causing endometriosis.

A "conditionally essential" nutrient, L-carnitine, as a primary suspect in endometriosis.

L-carnitine, when administered to young female mice, has been shown to induce a pathologic condition resembling human endometriosis accompanied by a marked degree of infertility. Thus, the use of this nutrient by young women may be a potential risk factor responsible for the onset of endometriosis at a later stage of their lives.

Characterization of CD25-positive T cells during syngeneic pregnancy: production of stimulatory class II MHC molecules.

Various studies demonstrate that immunosuppression vis-à-vis paternal alloantigens may play a role for successful pregnancy. However, if this theory is true, the question that remains unanswered is how do syngeneic pregnancies manage to produce viable embryos. In allogeneic murine pregnancies immunosuppression is mediated by regulatory CD25(+)/Foxp3/CTLA-4 T cells.

Recent knowledge and new pharmaceutical products in potential alleviation of endometriosis.

Endometriosis is so far considered as an incurable inflammatory disease. The ectopical implants of endometrial cells proliferate, increase in size and thereafter bleed following the menstrual cycle. The accumulated blood aggravates the situation by developing into cysts which, depending on the place, size and number, in most cases increase pain.

Characterization of intracellular HLA-DR, DM and DO profile in K562 and HL-60 leukemic cells.

Surface class-II antigen expression fires-up the antigen presentation process and development of immune response. The absence of surface HLA-DR is used in various systems to avoid immune recognition. Most leukemic cells use such mechanism to escape immune surveillance.

Periodic symmetric functions, serial addition, and multiplication with neural networks.

This paper investigates threshold based neural networks for periodic symmetric Boolean functions and some related operations. It is shown that any n-input variable periodic symmetric Boolean function can be implemented with a feedforward linear threshold-based neural network with size of O(log n) and depth also of O(log n), both measured in terms of neurons. The maximum weight and fan-in values are in the order of O(n).

Elementary function generators for neural-network emulators.

Piece-wise first- and second-order approximations are employed to design commonly used elementary function generators for neural-network emulators. Three novel schemes are proposed for the first-order approximations. The first scheme requires one multiplication, one addition, and a 28-byte lookup table.

Endometriosis and infertility: a multi-cytokine imbalance versus ovulation, fertilization and early embryo development.

Endometriosis is tightly linked to infertility which is manifested at very early or more advanced stages of the gestational cycle. Alteration on the production of a great number of cytokines/growth factors can be accused for problems on ovum maturation, fertilization or implantation. Yet, macroscopically these stages are characterized by the inability of conception.

Constitutive or induced elevated levels of L-carnitine correlate with the cytokine and cellular profile of endometriosis.

During the past decade, accumulated evidence indicates an association between endometriosis and an alteration of humoral and cell-mediated immunity. While the role of L-carnitine in the regulation of energy metabolism is well established, it is only recently that L-carnitine has been recognized to modify the immune response in mice after in vitro or in vivo treatment. The present study has examined whether administration of L-carnitine to young female mice alters the percentage of immune cells in peritoneal exudates and the uterus as well as the levels of IFN-gamma, TNF-alpha, IL-2, IL-4, IL-6, VEGF, GM-CSF and IGF-I in blood serum, peritoneal fluid and supernatants of uterine cultured cells as tested by immunofluorescence or ELISA techniques, respectively, leading to a pathological disorder resembling human endometriosis.