Publications by authors named "Vasiliy D Potapov"
Endolysin-based therapeutics are promising antibacterial agents and can successfully supplement the existing antibacterial drugs array. It is specifically important in the case of Gram-negative pathogens, e.g.
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- The study investigates the virulence of two clinical strains of the Beijing genotype associated with drug-resistant tuberculosis in C57BL/6 mice, highlighting their correlation to global health issues.
- Strains 267/47 (pre-XDR) and 120/26 (MDR) showed differing survival rates in infected mice, with 10% and 40% survival, respectively, compared to 70% for the H37Rv strain.
- Comparison of immune responses revealed significant differences in cytokine gene expression, particularly with downregulated pro-inflammatory and anti-inflammatory genes in mice infected with the more virulent strain 267/47.
Correction for Kuzina et al., "Carriage of Capsular Serotype K1 Klebsiella pneumoniae Sequence Type 23 Strains in Healthy Microbiology Laboratory Staff in Russia".
Microbiol Resour Announc
July 2021
Surfaces of implanted medical devices are highly susceptible to biofilm formation. Bacteria in biofilms are embedded in a self-produced extracellular matrix that inhibits the penetration of antibiotics and significantly contributes to the mechanical stability of the colonizing community which leads to an increase in morbidity and mortality rate in clinical settings. Therefore, new antibiofilm approaches and substances are urgently needed.
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- The Central Asia Outbreak (CAO) clade is a serious public health concern in Central Asia, featuring strains that are both multidrug-resistant and highly transmissible.
- Research using C57Bl/6 mice revealed that those infected with the drug-resistant Rostov strain died within 48 days, while a more traditional strain, H37Rv, had better survival rates.
- Analysis of lung and liver tissues showed different pathological effects from the two strains, with the Rostov strain causing a higher bacterial load and severe physical depletion in infected mice.
The use of recombinant endolysins is a promising approach for antimicrobial therapy capable of counteracting the spread of antibiotic-resistant strains. To obtain the necessary biotechnological product, diverse peptide tags are often fused to the endolysin sequence to simplify enzyme purification, improve its ability to permeabilize the bacterial outer membrane, etc. We compared the effects of two different types of protein modifications on endolysin LysECD7 bactericidal activity in vitro and demonstrated that it is significantly modulated by specific permeabilizing antimicrobial peptides, as well as by widely used histidine tags.
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