Publications by authors named "Vasilevich I"
The design of novel wound dressings for chronic wound treatment is still of great importance. One of the promising approaches is application of mesenchymal stem cells (MSCs), immobilized on a flexible polymer film, for healing. In this study, blended films based on polyvinyl alcohol (PVA) and pectin with different component ratio have been prepared by solution casting method and evaluated.
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- The study focused on creating ultrathin poly(L-lactic acid) (PLLA) films using a spin-coating technique and examined their physical properties like thickness and transparency.
- By adjusting polymer concentration and humidity, researchers were able to control the film's surface structure, producing either microporous or flat films.
- The resulting PLLA films supported the growth of mesenchymal stem cells and fibroblasts, demonstrated effective drug entrapment and release, making them promising materials for regenerative medicine in treating chronic wounds.
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- Layer-by-Layer assembled polyelectrolyte films utilize negatively charged biopolymers and positively charged polysaccharides to influence cell attachment and behavior on surfaces.
- The study focused on the properties of these films, particularly those containing chitosan, which exhibited linear growth, smooth surfaces, and viscoelastic characteristics, while films with polyethyleneimine showed increased rigidity and roughness.
- When tested with mesenchymal stem cells, chitosan films promoted effective cell adhesion and high viability, suggesting that the stiffness and surface properties of these films are crucial for tissue engineering applications.
The human adipose-derived mesenchymal stem cells was shown to be hepatic differentiated under influence of inductor factors. The differentiation of MSCs was induced by fibroblast growth factor-4, hepatocyte growth factor, oncostatin M and dexamethasone. The influence of quercetin on the hepatic differentiation of MSCs in culture was investigated.
View Article and Find Full Text PDFThe levels and rates of accumulation of porphyrins in lymphoid cells and bone marrow cells treated with exogenous 5-aminolaevulinic acid (ALA) were studied. The dependence of the quantity of porphyrins accumulated in cell on ALA concentrations in the medium had maximum at 0.7-1.
View Article and Find Full Text PDFThe influence of cytokine LIF (Leukemia Inhibitory Factor) on the viability, and proliferation of mouse R1 line embryonic stem cells (ESC) and their distribution by cell cycle stages has been investigated. LIF (5-20 ng/ml) increased growth of colonies and maintained high proliferative and pluripotent properties of R1. LIF was also involved into the inhibition of spontaneous cell differentiation and apoptotic cell death; it also decreased the rations of S/G2+M cell cycle and doubling-time of cell population.
View Article and Find Full Text PDFPeripheral lymphocytes (PL) were studied in 63 patients with pulmonary tuberculosis and 28 donors. The study revealed the elevated levels of lipid peroxidation products, altered membrane potential, and diminished PL viability in patients with pulmonary tuberculosis as compared with healthy donors. Analysis of the time dependences of cell viability and production of active tuberculosis products after endogenous porphyrin-photosensitized exposure suggests that the lymphocytes from patients with pulmonary tuberculosis are more exposed to the damaging effect of free radicals than the cells from healthy individuals.
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