Aim: The aim of the present study was to evaluate the bactericidal effects of different disinfection methods against microbial biofilms grown in root canals.
Methods: A total of 300 freshly-extracted human teeth were infected with microbial pathogens. The biofilm formation and the effects of laser therapy, photodynamic therapy (PDT), iontophoresis, and disinfection with irrigating solutions were evaluated by counting the generations of microbial cells in the samples of root canals and by scanning electron microscopy.