Productivity of Concentration-Dependent Conversion of Substitutional Nitrogen Atoms into Nitrogen-Vacancy Quantum Emitters in Synthetic-Diamond by Ultrashort Laser Pulses.

Tightly focused 515-nm, 0.3-ps laser pulses modify in a laser filamentation regime the crystalline structure of an Ib-type high-pressure, high-temperature (HPHT) synthesized diamond in a thin-plate form. The modified microregions (micromarks) in the yellow and colorless crystal zones, possessing different concentrations of elementary substitutional nitrogen (N) impurity atoms (C-centers), exhibit their strongly diminished local IR absorption (upon correction to the thickness scaling factor).

[Sulfonium derivatives of thioxanthenone, a new class of photodetritylating agents for microarray oligonucleotide synthesis].

The usability of a new class of photo acids, namely, sulfonium hexaphosphates based on thioxanthenone, for the removal of the dimethoxytrityl protective group in the process of oligonucleotide synthesis has been studied in order to search for new detritylating agents for microarray oligodeoxyribonucleotide synthesis. 2,4-Diethyl-9-oxo-10-(4-heptyloxyphenyl)-9H-thioxanthenium hexafluorophosphate has been successfully used for the solid-phase synthesis of (dT)(10).

[Rapid identification of mycobacteria using laser fluorescence].

Aim: To develop rapid method of identification of mycobacteria based on laser fluorescence.

Materials And Methods: Characteristics of laser-induced fluorescence of 19 bacteria species, including 17 species of mycobacteria, were studied. Identification of microorganisms was performed using measurement of spectral-fluorescent characteristics.

[Antimicrobial activity determination of the preparations used in comprehensive treatment of patients with parodontitis].

Determination of antimicrobic activity of the preparations of Metrogyl denta, Cholisal and Solcoseryl on the complex culture of microorganisms employing the developed procedure was carried out. Degree of the antimicrobic action manifestation of each preparation was revealed.

[Modelling of photoinducted diffusion of antimicrobial preparations in gingival tissues and face skin].

The study was made of laser radiation influence upon diffusion speed of medicines applied on tissue surface. Photophoresis use allow to increase diffusion speed of medicines applied on parodontal tissues and face skin during photophoresis process.

[Frequency of RET/PTC rearrangement and somatic BRAF mutation in papillary thyroid cancer].

Frequency of RET/PTC rearrangement and somatic BRAF mutation was investigated in patients with papillary thyroid cancer (PTC) vis-a-vis relevant demographic and clinico-pathological features. The study group included 76 patients with a female/male ratio of 4.8:1; mean age - 45.

[Age- and sex-related differences in sensitivity to hepatotoxic action of estragole in mice].

As determined by blood activity of alanine- and aspartate aminotransferases, intraperitoneal injection of estragole in subcarcinogenic dose 300 mg/kg does not damage the liver of suckling off-spring of both sexes and of adult SWR/J males but drastically damages it in mature females of this strain. Castration only slightly decreases the resistance of males to hepatotoxic action of estragole but significantly increases it in females; exogenous administration of estradiole benzoate to castrated males decreases their resistance to the hepatotoxin, whereas administration of testosterone propionate to ovariectomyzed females does not elevate it. Morphologically, estragole damages the same number of liver cells in females and males, but in males it induces mostly hydropic degenerative, and in females--necrotic changes of hepatocytes.

[Abnormal methylation of p16/CDKN2A AND p14/ARF genes GpG Islands in non-small cell lung cancer and in acute lymphoblastic leukemia].

Multiplex methylation-sensitive PCR and methylation-specific PCR were employed in studying the methylation of CpG islands in the p16/CDKN2A and p14/ARF promoter and the first exon regions in non-small cell lung cancer (54 samples) and acute B-cell lymphoblastic leukemia (61 samples). Differences in methylation were detected between types of neoplasia as well as between CpG islands studied within the same types of tumors. High level of the p16/CDKN2A first exon CpC island methylation was revealed in non-small cell lung cancer (68%) and in acute B-cell lymphoblastic leukemia (55%) and the CpG island of p14/ARF first exon was nonmethylated in these types of tumors.

[Molecular analysis of structural abnormalities in papillary thyroid carcinoma gene].

Rearrangements of the RET proto-oncogene (RET/PTC) and BRAF gene mutations are the major genetic alterations in the etiopathogenesis of papillary thyroid carcinoma (PTC). We have analyzed a series of 118 benign and malignant follicular cell-derived thyroid tumors for RET/PTC rearrangements and BRAF gene mutations. Oncogenic rearrangements of RET proto-oncogene was revealed by semiquantitative RT-PCR of simultaneously generated fragments corresponding to tyrosine kinase (TK) and extracellular RET domains.

[Diagnostics for epigenetic pathology in hereditary and oncologic diseases].

The review considers the epigenetic defects and their diagnostics in several hereditary disorders and tumors. Aberrant methylation of the promoter or regulatory region of a gene results in its functional inactivation, which is phenotypically similar to structural deletion. Screening tests were developed for Prader-Willi, Angelman, Wiedemann-Beckwith, and Martin-Bell syndromes and mental retardation FRAXE.

[Diagnosis and treatment of syndrome of multiple endocrine neoplasia type 2].

Since 1969 to 2000 twenty one patients from 16 families with syndrome of multiple endocrine neoplasia (MEN) type 2 were examined. Medullary cancer of the thyroid gland (MCTG) was diagnosed in 18 patients, pheochromocytoma--in 15 (in 13 of them--two-sided), primary hyperparathyroidism--in 2. In 9 patients from 5 families syndrome MEN 2 was confirmed genetically (mutation in codon 634 of 11th exon RET in 7 patients with MEN 2a and in codon 918 in 2 patients with MEN 2b).

[A computerized expert system for the diagnosis and prognosis of the course of acute odontogenic inflammatory diseases (DIAPRO)].

A computer-aided expert system for diagnosis and prediction of the course of acute odontogenic inflammatory diseases DIAPRO is created. Based on commonly used laboratory and clinical parameters, it permits a long-distance diagnosis of acute maxillofacial inflammations and their complications and provides data for 13 signs of patient's status.

[A set of instruments for laser choledochotomy and papillosphincterotomy].

Development and series production of a special instrument for realization of extrahepatic biliary tract surgery with the use of laser surgery units Skalpel-1, Romashka-1 and Romashka-2 is planned for 1987. It will expand the possibilities of biliary tract laser surgery and increase the effect of surgical interventions.