Structural characterization of a sulfated polysaccharide from Gracilariadomingensis and potential anti-inflammatory and antinociceptive effects.

Seaweeds are natural sources of sulfated polysaccharides (SPs), biopolymers with remarkable pharmacological properties, including biological actions capable of attenuating components of the inflammatory process such as edema, cytokines, cell migration and pain. Our results confirm that SPs obtained from Gracilaria domingensis (SP-GD) are agarans, primarily composed of residues of β-d-galactopyranose 6-sulfate and 3,6-anhydro-α-l-galactopyranose. Specifically, SP-GD at a dose of 10 mg/kg was effective in significantly reducing paw edema induced by carrageenan or histamine, serotonin, bradykinin, 48/80 and prostaglandin E2.

Effects of maternal preconception high-fat diet on the fertility of dams and on the somatic parameters and reflex ontogeny of their male offspring.

Background: Female consumption of a high-fat diet (HFD) may cause fertility issues and affects offspring development.

Objective: Evaluate the acute maternal preconception intake of a HFD on the fertility and reproduction parameters of breeding females; and on the somatic parameters and reflex ontogeny of male offspring.

Methods: Twenty-four rats were randomized into control (PC; n=12) and high-fat diet group (PHF; n=12) that consumed their respective diets during the 23-day preconception period.

Phase-transition of hydrophobic dipeptide l-phenylalanyl-l-alanine under high pressure via Raman spectroscopy.

The study conducted on l-Phenylalanyl-l-Alanine (CHNO) dehydrated crystals under high-pressure conditions up to 7.0 GPa using Raman spectroscopy revealed significant modifications in both lattice and internal modes. Particularly, alterations were observed in modes associated with carbonyl and NH functionalities around 1600 cm.

In silico analysis of aptamer-RNA conjugate interactions with human transferrin receptor.

The human transmembrane protein Transferrin Receptor-1 is regarded as a promising target for the systemic delivery of therapeutic agents, particularly of nucleic acid therapeutics, such as short double stranded RNAs. This ubiquitous receptor is involved in cellular iron uptake, keeping intracellular homeostasis. It is overexpressed in multiple cancer cell types and is internalized via clathrin-mediated endocytosis.

Hydrodynamic Evaluation of a Filtering Hydrocyclone for Solid Particle/Water Separation.

A conventional hydrocyclones is a versatile equipment with a high processing capacity and low maintenance cost. Currently, several studies aim to alter the typical structure of the conventional hydrocyclone in order to modify its performance and purpose. For this, filtering hydrocyclones have emerged, where a porous membrane replaces the conic or cylindrical wall.

Educational interventions for the prevention and management of adverse events following immunisation: a systematic review.

This study investigated educational interventions for the prevention and management of adverse events following immunisation. This a systematic review was conducted by examining observational studies, with no restriction as to language or year, registered in PROSPERO with the identifier CRD42022313144 and by searching the MEDLINE, LILACS, Embase, CINAHL and Scopus databases. Two researchers selected the studies, extracted the data and assessed the risk of study bias; disagreements were resolved by a third researcher.

Neuro-Immunomodulatory Potential of Nanoenabled 4D Bioprinted Microtissue for Cartilage Tissue Engineering.

Cartilage defects trigger post-traumatic inflammation, leading to a catabolic metabolism in chondrocytes and exacerbating cartilage degradation. Current treatments aim to relieve pain but fail to target the inflammatory process underlying osteoarthritis (OA) progression. Here, a human cartilage microtissue (HCM) nanoenabled with ibuprofen-loaded poly(lactic-co-glycolic acid) nanoparticles (ibu-PLGA NPs) is 4D-bioprinted to locally mitigate inflammation and impair nerve sprouting.

Polymorphisms and haplotypes of and are associated with susceptibility and survival in patients with fibrotic hypersensitivity pneumonitis.

Introduction And Objectives: Hypersensitivity pneumonitis (HP) is an interstitial lung disease with diverse clinical features that can present a fibrotic phenotype similar to idiopathic pulmonary fibrosis (IPF) in genetically predisposed individuals. While several single nucleotide polymorphisms (SNPs) have been associated with IPF, the genetic factors contributing to fibrotic HP (fHP) remain poorly understood. This study investigated the association of and variants with susceptibility, clinical presentation and survival in Portuguese patients with fHP.

Effect of the Flavonoid Rutin on the Modulation of the Myenteric Plexuses in an Experimental Model of Parkinson's Disease.

Recent discoveries have shown that enteric glial cells play an important role in different neurodegenerative disorders, such as Parkinson's disease (PD), which is characterized by motor dysfunctions caused by the progressive loss of dopaminergic neurons in the substance nigra pars compacta and non-motor symptoms including gastrointestinal dysfunction. In this study, we investigated the modulatory effects of the flavonoid rutin on the behavior and myenteric plexuses in a PD animal model and the response of enteric glia. Adult male Wistar rats were submitted to stereotaxic injection with 6-hydroxydopamine or saline, and they were untreated or treated with rutin (10 mg/kg) for 14 days.

The annonalide diterpene extracted from Casimirella ampla (Miers) reduces inflammatory and antinociceptive events in general models of inflammation.

Ethnopharmacological Relevance: The plants of the genus Casimirella ampla (Miers) (C. ampla) are extensively used in folk medicine. For a long time, rural communities have been using extracts from its roots for food and therapeutic purposes.

Gastroprotective activity and physicochemical analysis of carboxymethylated gum from Anadenanthera colubrina.

Biotechnological advancements require the physicochemical alteration of molecules to enhance their biological efficacy for the effective treatment of gastric ulcers. The study aimed to produce a polyelectrolytic compound from red angico gum (AG) by carboxymethylation, evaluate its physicochemical characteristics and investigate gastric protection against ethanol-induced ulcers. AG and carboxymethylated angico gum (CAG) were characterized by Fourier transform infrared spectroscopy, determination of the degree of substitution and gel permeation chromatography (GPC) and C NMR techniques.

Optimal 2D audio features estimation for a lightweight application in mosquitoes species: Ecoacoustics detection and classification purposes.

Mosquitoes are the vector of diseases that kill more than one million people per year worldwide. Surveillance systems are essential for understanding their complex ecology and behaviour. This is fundamental for predicting disease risk caused by mosquitoes and formulating effective control strategies against mosquito-borne diseases such as malaria, dengue, and Zika.

Visceral Leishmaniasis Revealing Undiagnosed Inborn Errors of Immunity.

Visceral Leishmaniasis (VL) is a potentially fatal disease and may be associated with primary or acquired immunodeficiencies. There are few reports, in the literature, of inborn errors of immunity. Here, we report two cases of VL as a marker of inborn errors of immunity, namely, GATA2 and RAB27A deficiency.

Real-Life Data on Hydroxychloroquine or Chloroquine with or Without Azithromycin in COVID-19 Patients: A Retrospective Analysis in Brazil.

Background: Despite no evidence showing benefits of hydroxychloroquine and chloroquine with or without azithromycin for COVID-19 treatment, these medications have been largely prescribed in Brazil.

Objectives: To assess outcomes, including in-hospital mortality, electrocardiographic abnormalities, hospital length-of-stay, admission to the intensive care unit, and need for dialysis and mechanical ventilation, in hospitalized COVID-19 patients who received chloroquine or hydroxychloroquine, and to compare outcomes between those patients and their matched controls.

Methods: A retrospective multicenter cohort study that included consecutive laboratory-confirmed COVID-19 patients from 37 Brazilian hospitals from March to September 2020.

Impact of Treatment with Ustekinumab on Severe Infections in a Patient with Uncontrolled Psoriasis and Late-Onset Combined Primary Immunodeficiency: Case Report.

A 35-year-old man with a late-onset combined immunodeficiency (LOCID) variant of common variable immunodeficiency, severe plaque psoriasis, psoriatic arthritis, and Crohn's disease was attended in the Regional Hospital of Presidente Prudente and HC-FMUSP, São Paulo, Brazil. Anti-IL-12/IL-23 (ustekinumab) monoclonal antibody was prescribed due to the failure of other treatments (phototherapy, oral acitretin) for psoriasis and a Psoriasis Area Severity Index >10. We evaluated the impact of treatment with ustekinumab on severe infectious diseases in a patient with uncontrolled psoriasis and LOCID followed for 8 years.

Organic selenium and vitamin E for gilts and sows bred in equatorial climate.

The aim of this study was to evaluate the dietary supplementation of organic selenium (Se) and vitamin E for sows from 1st and 2nd parity order, without adiabatic cooling on physiological parameters, reproductive performance, milk composition, litter performance and blood concentrations of antioxidant enzymes. A total of 96 sows were allotted in a 4 × 2 completely factorial design, with 4 experimental groups and 2 parity orders (1st and 2nd). The experimental groups consisted of: ACCon-sows receiving adiabatic cooling and no dietary supplementation of organic Se and vitamin E; WACCon-sows without adiabatic cooling and no dietary supplementation of organic Se and vitamin E; WACSe-sows without adiabatic cooling with dietary supplementation of 0.

In situ Raman spectroscopy and synchrotron X-ray diffraction studies on maleic acid under high pressure conditions.

Maleic acid was studied by Raman spectroscopy and powder synchrotron X-ray diffraction (XRD) under high pressure conditions by using a diamond anvil cell. The Raman spectroscopy measurements were performed from ambient pressure up to 9.2 GPa in the 100-3200 cm spectral range.

A model for preservation of thymocyte-depleted thymus.

DiGeorge syndrome is a disorder caused by a microdeletion on the long arm of chromosome 22. Approximately 1% of patients diagnosed with DiGeorge syndrome may have an absence of a functional thymus, which characterizes the complete form of the syndrome. These patients require urgent treatment to reconstitute T cell immunity.

A brazilian nationwide multicenter study on deficiency of deaminase-2 (DADA2).

Introduction: The deficiency of ADA2 (DADA2) is a rare autoinflammatory disease provoked by mutations in the ADA2 gene inherited in a recessive fashion. Up to this moment there is no consensus for the treatment of DADA2 and anti-TNF is the therapy of choice for chronic management whereas bone marrow transplantation is considered for refractory or severe phenotypes. Data from Brazil is scarce and this multicentric study reports 18 patients with DADA2 from Brazil.

Risk assessment of ionizing radiation and radiological thresholds to compound an environmental baseline for the unconventional gas industry.

The exploration of unconventional hydrocarbons may be very effective in promoting economic development and confronting energy crisis around the world. However, the environmental risks associated with this practice might be an impediment if not adequately dimensioned. In this context, naturally occurring radioactive materials and ionizing radiation are sensitive aspects in the unconventional gas industry that may compromise the environmental sustainability of gas production and they should be properly monitored.

Factors shaping the gut microbiome of five species of lizards from different habitats.

Background: Host-gut microbiota interactions are complex and can have a profound impact on the ecology and evolution of both counterparts. Several host traits such as systematics, diet and social behavior, and external factors such as prey availability and local environment are known to influence the composition and diversity of the gut microbiota.

Methods: In this study, we investigate the influence of systematics, sex, host size, and locality/habitat on gut microbiota diversity in five lizard species from two different sites in Portugal: and , living in syntopy in a rural area in northern Portugal (Moledo); the invasive and the native , living in sympatry in an urbanized environment (Lisbon); and the invasive also living in an urban area (Lisbon).

Raman study of pressure-induced phase transitions in imidazolium manganese- hypophosphite hybrid perovskite.

By using Raman spectroscopy, we demonstrate that [IM]Mn(HPOO) is a highly compressible material that undergoes three pressure-induced phase transitions. Using a diamond anvil cell we performed high-pressure experiments up to 7.1 GPa, using paraffin oil as the compression medium.

Chitosan 3D scaffolds with resolvin D1 for vertebral arthrodesis: a pilot study.

Purpose: Over the last years, the number of vertebral arthrodesis has been steadily increasing. The use of iliac crest bone autograft remains the "gold standard" for bone graft substitute in these procedures. However, this solution has some side effects, such as the problem of donor site morbidity indicating that there is a real need for adequate alternatives.

Salivary biomarkers present in patients with periodontitis without clinical distinction: findings from a meta-analysis.

Background: A new classification for periodontitis has been adopted in clinical practice. However, there are still discussions regarding this new classification and difficulties in its adoption, both by professionals and researchers. Thus, this study aimed to evaluate which salivary biomarkers are present in periodontitis, following the new classification of periodontal diseases through meta-analysis.

BAROS PROTOCOL IN A UNIVERSITY HOSPITAL: WHAT IS THE IMPORTANCE IN THE POSTOPERATIVE RESULTS OF BARIATRIC SURGERY?

Background: Although bariatric surgery is highly effective for the treatment of obesity and its comorbidities, preoperative weight loss has an impact on its results.

Aims: The aim of this study was to correlate preoperative weight loss with the outcome of bariatric surgery using the Bariatric Analysis and Reporting Outcome System scores.

Methods: This is a cross-sectional, observational study with 43 patients undergoing bariatric surgery that compared a group of 25 patients with a percentage of preoperative excess weight loss ³8% with a group of 18 patients with a percentage of preoperative excess weight loss <8% or with weight gain.