Decoding the molecular properties of mycobacteriophage D29 Holin provides insights into Holin engineering.

Holins are bacteriophage-encoded small transmembrane proteins that determine the phage infection cycle duration by forming non-specific holes in the host cell membrane at a specific time post-infection. Thus, Holins are also termed as "Protein clocks". Holins have one or more transmembrane domains, and a charged C-terminal region, which, although conserved among Holins, has not yet been examined in detail.

Deciphering the Role of Holin in Mycobacteriophage D29 Physiology.

In the era of antibiotic resistance, phage therapy is gaining attention for the treatment of pathogenic organisms such as . The selection of phages for therapeutic purposes depends upon several factors such as the host range that a phage can infect, which can be narrow or broad, time required for the host cell lysis, and the burst size. Mycobacteriophage D29 is a virulent phage that has the ability to infect and kill several slow- and fast-growing mycobacterial species including the pathogenic .