Cross-family small GTPase ubiquitination by the intracellular pathogen .

The intracellular bacterial pathogen manipulates eukaryotic host ubiquitination machinery to form its replicative vacuole. While nearly 10% of 's arsenal of ~330 secreted effector proteins have been biochemically characterized as ubiquitin ligases or deubiquitinases, a comprehensive measure of temporally resolved changes in the endogenous host ubiquitinome during infection has not been undertaken. To elucidate how hijacks ubiquitin signaling within the host cell, we undertook a proteome-wide analysis of changes in protein ubiquitination during infection.