Publications by authors named "Variya Nemidkanam"
Purpose: extracellular vesicles (KPEVs) have been reported as promising nanovesicles for drug delivery. This study aimed to load clarithromycin (CLA) into KPEVs (KPEVS-CLA) and determine the physical properties, drug-releasing efficiency, gastric cell uptake, anti- activities, and anti-inflammatory responses in comparison with free CLA and KPEVs.
Methods: The size and surface charge of KPEVs-CLA were evaluated using dynamic light scattering and visualized using a transmission electron microscope.
Plant-derived extracellular vesicles (EVs) are a promising candidate for nanomedicine delivery due to their bioactive cargos, high biocompatibility to human cells, biodegradability, low cytotoxicity, and potential for large-scale production. However, the research on EVs derived from medicinal plants is very limited. In this study, Kaempferia parviflora extracellular vesicles (KPEVs) were isolated by differential and sucrose density gradient centrifugation, and their size, morphology, and surface charge were characterized using transmission electron microscopy and dynamic light scattering.
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- Kaempferia parviflora (KP) has historically been used in Thai medicine to treat gastrointestinal issues, specifically targeting inflammation caused by Helicobacter pylori, which can lead to gastric diseases and cancer.
- A study evaluated the effects of a crude ethyl acetate extract of KP (CEAE-KP) on AGS gastric cancer cells and found that it reduced cell viability in a dose- and time-dependent manner, with no toxicity at lower concentrations (8-16 μg/ml).
- CEAE-KP significantly decreased interleukin-8 (IL-8) production and neutrophil chemotaxis, suggesting its potential as an effective alternative treatment to reduce inflammation from H. pylori infections.