Role of AxyABM overexpression in acquired resistance in Achromobacter xylosoxidans.

Background: Acquired antimicrobial resistance among Achromobacter isolates from cystic fibrosis (CF) patients is frequent. Data concerning the mechanisms involved are scarce. The role of the AxyXY-OprZ and AxyEF-OprN Resistance Nodulation Division (RND) efflux systems has been demonstrated, but not that of AxyABM.

Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Rapid Detection of Isolates Belonging to the Epidemic Clones Achromobacter xylosoxidans ST137 and Achromobacter ruhlandii DES from Cystic Fibrosis Patients.

spp. are increasingly reported among cystic fibrosis patients. Genotyping requires time-consuming methods such as multilocus sequence typing or pulsed-field gel electrophoresis.

Mobilisation of plasmid-mediated bla gene cassette into distinct genomic islands of Proteus mirabilis after ceftazidime exposure.

Objectives: We sought to integrate a VEB-1-encoding gene cassette into the integron of the MDR region of genomic islands (GIs) harboured by Proteus mirabilis strains after antibiotic exposure.

Methods: An IncP1 plasmid from Achromobacter xylosoxidans carrying the cassette array dfrA14-bla-aadB was introduced by conjugation into five strains of P. mirabilis: PmBRI, PmABB, PmSCO and Pm2CHAMA harbouring Salmonella GI 1 and PmESC harbouring Proteus GI 1.

Fluoroquinolone resistance in Achromobacter spp.: substitutions in QRDRs of GyrA, GyrB, ParC and ParE and implication of the RND efflux system AxyEF-OprN.

Background: Achromobacter are emerging pathogens in cystic fibrosis patients. Mechanisms of resistance to fluoroquinolones are unknown in clinical isolates. Among non-fermenting Gram-negative bacilli, fluoroquinolone resistance is mostly due to amino acid substitutions in localized regions of the targets (GyrA, GyrB, ParC and ParE) named QRDRs, but also to efflux.

Two New SGI1-LK Variants Found in Proteus mirabilis and Evolution of the SGI1-HKL Group of Genomic Islands.

Integrative mobilizable elements belonging to the SGI1-H, -K, and -L genomic island 1 (SGI1) variant groups are distinguished by the presence of an alteration in the backbone (IS replaces 2.8 kb of the backbone extending from within [S005] to within S009). Members of this SGI1-HKL group have been found in serovars and in Two novel variants from this group, designated SGI1-LK1 and SGI1-LK2, were found in the draft genomes of antibiotic-resistant isolates from two French hospitals.

16S rRNA PCR on clinical specimens: Impact on diagnosis and therapeutic management.

Objective: 16S rRNA PCR (16S PCR) performed on clinical samples contributes to bacterial identification in cases of negative culture due to an antibiotic therapy. Sensitivity of the 16S PCR is low (19-42%). Little data is available on its impact on the management of patients.

[Care pathway and life course of drug-addicted patients].

Placing drug addicts, notably heroin addicts, at the centre of their care project, itself part of a life project, is the objective of the Le Lac d'Argent association in Annecy. Caregivers have been able to reflect on their practices and help to fight against preconceived ideas. This article presents the experience of a global support approach.

Detection of Achromobacter xylosoxidans in hospital, domestic, and outdoor environmental samples and comparison with human clinical isolates.

Achromobacter xylosoxidans is an aerobic nonfermentative Gram-negative rod considered an important emerging pathogen among cystic fibrosis (CF) patients worldwide and among immunocompromised patients. This increased prevalence remains unexplained, and to date no environmental reservoir has been identified. The aim of this study was to identify potential reservoirs of A.