Morbidly adherent placenta as a cause of peripartum hysterectomy in the Slovak Republic in the years 2012-2020.

Objective: The main aim of this study was to analyze the cases of peripartum hysterectomy associated with morbidly adherent placenta in the Slovak Republic.

Materials And Methods: Cases of morbidly adherent placenta managed by peripartum hysterectomy in the Slovak Republic between January 2012 and December 2020 were retrospectively analyzed. Data were obtained from the standardized anonymous questionnaires.

Modulatory Effect of Beneficial Enterococci and Their Enterocins on the Blood Phagocytes in Murine Experimental Trichinellosis.

Bacteriocins (enterocins) represent a new therapeutic strategy in various intestinal and non-intestinal infections. In antiparasitic defence, an oxidative inflammation of phagocytes is effective in destroying new-born larvae. The strains CCM8558 and ED26E/7 and their enterocins, enterocin M and a durancin-like enterocin, respectively, were administered daily, and mice were then infected with larvae on the seventh day of treatment.

Severe maternal morbidity requiring intensive care units admission in the Slovak Republic - a 9-year population based study.

Objective: Analysis of life-threatening maternal morbidities, the condition of which required subsequent treatment in Intensive Care Units (ICU) in the Slovak Republic in the years 2012-2020.

Methodology: Retrospective analysis of 655 identified cases of mothers admitted to the intensive care units out of 436,136 births. The reasons for the transport were divided into nine categories: peripartum bleeding, hypertensive diseases, thromboembolism, cardiovascular diseases, sepsis/severe infections, metabolic diseases, complications of anaesthesiology, gastroenterological problems and others.

Comparative Analysis of Methods for Somatic Cell Counting in Cow's Milk and Relationship between Somatic Cell Count and Occurrence of Intramammary Bacteria.

The aim of this study was to compare three on-farm commercial methods for the indirect detection of subclinical mastitis in dairy cows: the California mastitis test (CMT), the Porta side somatic cell count milk test (Porta SCC), and the DeLaval cell counter (DCC), with the Fossomatic cell count (FSCC), and to evaluate the relationship between the determined somatic cell count SCC and the occurrence of intramammary pathogens in the milk of dairy cows. A total of 284 sensory unchanged mixed milk samples, collected during the milking on a dairy farm, were analyzed in this study for somatic cell counts by the mentioned on-farm tests. Quarter milk samples ( = 583) from all the selected cows were cultured.

Peripartum hysterectomy: A 9-year population-based study in Slovakia.

Objective: To assess the incidence of peripartum hysterectomy (PH) in Slovakia. Additionally, we wanted to describe reasons for the procedure, associated clinical circumstances, and complications.

Methods: This was a descriptive, population-based study among women who underwent PH in Slovakia between January 2012 and December 2020.

Biofilm-Producing Ability of Obtained from Surfaces and Milk of Mastitic Cows.

This study was conducted to evaluate the incidence of mastitis in 153 dairy cows and to evaluate the kinetics of adhesion of isolates obtained from surfaces and milk in comparison with the reference strain (RS), CCM 4223. The surfaces of the floor, teat cup, and cow restraints were aseptically swabbed in three replicates (n = 27). Of the total number of infected cows (n = 43), 11 samples were found to be positive for , 12 samples tested positive for non-aureus staphylococci, 6 samples tested positive for spp.

Multi-cancer early detection test in symptomatic patients referred for cancer investigation in England and Wales (SYMPLIFY): a large-scale, observational cohort study.

Background: Analysis of circulating tumour DNA could stratify cancer risk in symptomatic patients. We aimed to evaluate the performance of a methylation-based multicancer early detection (MCED) diagnostic test in symptomatic patients referred from primary care.

Methods: We did a multicentre, prospective, observational study at National Health Service (NHS) hospital sites in England and Wales.

Effectiveness of sanitation regime in a milking parlour to control microbial contamination of teats and surfaces teat cups'.

Introduction And Objective: The major sources of bacterial contamination of raw milk are post-harvest manipulation; therefore the disinfection of teat and teat cups which decrease the bacterial load has a positive impact on minimizing new infection rates. The aim of the study was determination of the incidence of pathogens on investigated surfaces, evaluation of the effectiveness of sanitation regime in the reduction of surface microbial load, and determination of the effectiveness of mechanical cleaning of teats in a milking parlour for dairy cows.

Material And Methods: Samples from surfaces were taken by microbiological swabs using a sterile cotton swab from area of 5×2 cm.

Severe maternal morbidity in the Slovak Republic in the years 2012-2018.

Objective: Analysis of maternal morbidity in the Slovak Republic in the years 2012-2018.

Methods: The analysis of selected maternal morbidity data prospectively collected in the years 2012-2018 from all obstetric units in the Slovak Republic.

Results: In the years 2012-2018, incidence of severe peripartum bleeding was 2.

Caesarean section, vaginal instrumental delivery and perineal morbidity in the Slovak Republic in the years 2007-2018.

Objective: Analysis of caesarean section, vaginal instrumental deliveries and severe perineal morbidity in the Slovak Republic in the years 2007-2018.

Methods: The analysis of prospectively collected caesarean section and vaginal instrumental delivery data in the years 2007-2018, and episiotomies and severe perineal morbidity data in the years 2008-2018 from obstetrics hospitals in the Slovak Republic.

Results: Caesarean section rate progressively increased from 24.

Modulation of lymphocyte subpopulations in the small intestine of mice treated with probiotic bacterial strains and infected with Trichinella spiralis.

Aims: To study the local intestinal lymphocyte immunity in mice with trichinellosis affected by probiotic bacteria.

Methods And Results: Enterococcus faecium CCM8558, Enterococcus durans ED26E/7, Limosilactobacillus fermentum CCM7421 and Lactiplantibacillus plantarum 17 L/1 were administered daily (10  CFU ml ) and mice were infected with Trichinella spiralis (400 larvae) on the 7th day of treatment. T.

Effect of Ornithine Transcarbamylase (OTC) Deficiency on Pregnancy and Puerperium.

Ornithine transcarbamylase (OTC) deficiency is the most common inherited metabolic disorder in urea cycles with an incidence of 1:14,000 live births. Pregnancy, childbirth and the postpartum period are considered challenging for women with this hereditary metabolic disorder, with a risk of hyperammonemia, especially in the first week after delivery. In our article, we discuss severe hepatic failure, a pregnancy complication in an OTC deficient patient that has not previously been published.

Dairy Cows' Udder Pathogens and Occurrence of Virulence Factors in Staphylococci.

This study investigated 960 Slovak and Czech spotted cattle from four different conventional (non-organic) dairy herds located in Eastern Slovakia and Czechia during early lactation (14-100 days after calving). Dairy cows were examined clinically; milk from fore-stripping of each udder quarter was subjected to sensory examination and assessed by the California mastitis test (CMT), and laboratory analyses of bacterial pathogens in milk, including virulence factors, were conducted. Positive CMT scores (1-3) for one or more quarters were detected in 271 (28.

Antimicrobial Resistance of sp. Isolated from Sheep and Goat Cheeses.

This study aimed to calculate the proportion of antibiotic resistance profiles of , and isolated from traditional sheep and goat cheeses obtained from a selected border area of Slovakia with Hungary (region Slanské vrchy). A total of 110 sp. were isolated from cheese samples, of which 52 strains ( (12), (28), (12)) were represented.

Postpartum ovarian vein thrombosis: case report and review of literature.

Objective: An analysis of POVT (postpartum ovarian vein thrombosis) case, the importance of prompt diagnosis, antibiotic and anticoagulation therapy management with multidisciplinary team approach.

Design: A case report and literature review.

Setting: 1st Department of Gynaecology and Obstetrics, Faculty of Medicine Comenius University and University Hospital, Bratislava, Slovakia.

Cellular and humoral peritoneal immunity to Mesocestoides vogae metacestode infection in mice.

Background: Here, Mesocestoides (M.) vogae infection in mice is proposed as a suitable experimental model for studying the immunity in the peritoneal cavity of mice.

Methods: To investigate the kinetics of immune parameters in M.

Risk of exposure to acrylamide.

Objectives: Acrylamide is a toxic compound that can be found it both occupational and non-occupational environments. This study focuses on its sources and health effects of its exposure.

Methods: Adverse effects of acrylamide, especially carcinogenic, genotoxic, and teratogenic were reported in many studies conducted on animals.

Probiotic Bacteria can Modulate Murine Macrophage's Superoxide Production in Infection.

The effect of probiotic strains ( EF55, CCM7420, CCM8558, ED26E/7, CCM7421, 17L/1) on the production of superoxide anion (O ) in peritoneal macrophages of infected mice was examined. EF55 and CCM8558 strains increased the O production prior to parasitic infection,at the day7of application.A significant inhibition of the O production caused by infection on day 5 infection (p.

The International Network of Obstetric Survey System.

Objective: To informed about international surveillance network severe maternal morbidity and mortality - INOSS.

Design: Literature review.

Settings: 1st Department of Gynaecology and Obstetrics Faculty of Medicine, Comenius University and University Hospital, Bratislava, Slovak Republic.

Enteral lactoferrin to prevent infection for very preterm infants: the ELFIN RCT.

Background: Infections acquired in hospital are an important cause of morbidity and mortality in very preterm infants. Several small trials have suggested that supplementing the enteral diet of very preterm infants with lactoferrin, an antimicrobial protein processed from cow's milk, prevents infections and associated complications.

Objective: To determine whether or not enteral supplementation with bovine lactoferrin (The Tatua Cooperative Dairy Company Ltd, Morrinsville, New Zealand) reduces the risk of late-onset infection (acquired > 72 hours after birth) and other morbidity and mortality in very preterm infants.

Concentrations of selected toxic elements in ewe living near an environmentally loaded area of eastern part of Slovakia.

Introduction And Objective: Research focused on the monitoring of selected heavy metals in ewes' blood. Concentrations of selected toxic elements, lead, cadmium and zinc, in ewes living near an environmentally-loaded area, concerned 15 ewes (aged 3-4 years) in good physical condition, during the spring of 2014 and 2015 in the eastern part of Slovakia. The aim of the research was to determine the concentration of selected heavy metals and state the correlations of selected heavy metals in ewes' blood.

The role of CRP, PCT, IL-6 and presepsin in early diagnosis of bacterial infectious complications in paediatric haemato-oncological patients.

Bacterial infection is the most common complication in paediatric oncological patients during cancer treatment. A suitable tool for early prediction of unfavourable course of infection is still needed. We performed a prospective longitudinal observational study to evaluate of the role of serum biomarkers (C-reactive protein, procalcitonin, interleukin-6, presepsin) in the early diagnosis of bacteraemia (gram-negative versus gram-positive) in patients with haematological malignancies.

Directly created electrostatic micro-domains on hydroxyapatite: probing with a Kelvin Force probe and a protein.

Micro-domains of modified surface potential (SP) were created on hydroxyapatite films by direct patterning by mid-energy focused electron beam, typically available as a microprobe of Scanning Electron Microscopes. The SP distribution of these patterns has been studied on sub-micrometer scale by the Kelvin Probe Force Microscopy method as well as lysozyme adsorption. Since the lysozyme is positively charged at physiological pH, it allows us to track positively and negatively charged areas of the SP patterns.

In situ photoexcitation of silver-doped titania nanopowders for activity against bacteria and yeasts.

Photocatalytic and in situ microbial activity of the amorphous and annealed states of Ag-doped and un-doped titania were examined. Studies on their structure, morphology, composition, and the photo-absorption characteristics of these materials were performed. These results were correlated with the photocatalytic and microbial activity against methicillin resistant Staphylococcus aureus K324 (MRSA), methicillin susceptible S.

Application of peracetic acid and quarternary ammonium disinfectants as a part of sanitary treatment in a poultry house and poultry processing plant.

Hygiene and sanitation play a major role in any effective disease control programme for poultry production and processing premises. Various deficiencies in disinfection may induce that chains of infections are not broken from one stock to another. The present study investigated the efficacy of disinfection on a broiler farm and in a plant processing poultry from the investigated farm.