[ORF19.2286 Gene: Isolation and Purification of Deoxyhypusine Hydroxylase from the Human Pathogenic Yeast Candida albicans].

Candida albicans (C. albicans) is a fungal pathogen that causes infections of the wet body surfaces and the blood in immunocompromised patients or individuals with imbalanced microflora. Since the cases of clinically meaningful candidosis are on the rise, efficient С.

Solution structure of the N-terminal domain of the Staphylococcus aureus hibernation promoting factor.

Staphylococcus aureus hibernation promoting factor (SaHPF) is a 22,2 kDa protein which plays a crucial role in 100S Staphylococcus aureus ribosome formation during stress. SaHPF consists of N-terminal domain (NTD) that prevents proteins synthesis by binding to the 30S subunit at the P- and A-sites, connected through a flexible linker with a C-terminal domain (CTD) that keeps ribosomes in 100S form via homodimerization. Recently obtained 100S ribosome structure of S.

Reducing Humidity Response of Gas Sensors for Medical Applications: Use of Spark Discharge Synthesis of Metal Oxide Nanoparticles.

The application of gas sensors in breath analysis is an important trend in the early diagnostics of different diseases including lung cancer, ulcers, and enteric infection. However, traditional methods of synthesis of metal oxide gas-sensing materials for semiconductor sensors based on wet sol-gel processes give relatively high sensitivity of the gas sensor to changing humidity. The sol-gel process leading to the formation of superficial hydroxyl groups on oxide particles is responsible for the strong response of the sensing material to this factor.

ADAPTIVE MECHANISMS OF THE IMMUNE SYSTEM IN CHILDREN IN FAR NORTH.

This article is devoted to the probleme of modern Pediatrics pathology of the immune system in children with complicated viral infections. The aim of this study was to investigate the immune and cytokine status in children with complicated acute respiratory viral infections. The study included 100 children are often ill respiratory virus infections (more than 8 times per year) from age 0 to 7 years, and 30 children with upper respiratory tract infection; no more than 1 time per year-the control group.

[The accessibility of high-tech medical care of newborns with surgical pathology in conditions of Far North].

The actual stage of development of public health rendering of specialized medical care is based on principles of generality, accessibility, addressness, qualitativeness, and effectiveness. However, the problem of rendering specialized medical care to population is one of most critical targets in district centers and requires immediate solution. The main mean of resolving this problem is re-hospitalization of patient in more large-scale medical institutions.

[Contribution of L,D-carboxypeptidases in virulence of facultative intracellular pathogenic bacteria Listeria monocytogenes].

Aim: Evaluate influence of mutation of Listeria monocytogenes genes coding murein-tetrapeptide L,D-carboxypeptidase Lmo0028 and Lmo1638 on dynamics of infectious process and interaction of purified muropeptides with NOD1 receptor.

Materials And Methods: Wild type EGDe strain and recombinant strains GIMins1638 H GIMins0028 obtained on its basis by site-specific mutagenesis were used. Infectious process dynamics was studied on the model of intravenous infection of BALB/c mice.

Performance evaluation of two personal bioaerosol samplers.

In this study, the performance of two newly developed personal bioaerosol samplers for monitoring the level of environmental and occupational airborne microorganisms was evaluated. These new personal bioaerosol samplers were designed based on a swirling cyclone with recirculating liquid film. The performance evaluation included collection efficiency tests using inert aerosols, the bioaerosol survival test using viable airborne microorganism, and the evaluation of using non-aqueous collection liquid for long-period sampling.

Bactericidal effects of non-thermal argon plasma in vitro, in biofilms and in the animal model of infected wounds.

Non-thermal (low-temperature) physical plasma is under intensive study as an alternative approach to control superficial wound and skin infections when the effectiveness of chemical agents is weak due to natural pathogen or biofilm resistance. The purpose of this study was to test the individual susceptibility of pathogenic bacteria to non-thermal argon plasma and to measure the effectiveness of plasma treatments against bacteria in biofilms and on wound surfaces. Overall, Gram-negative bacteria were more susceptible to plasma treatment than Gram-positive bacteria.

Development of a personal bioaerosol sampler based on a conical cyclone with recirculating liquid film.

This article describes the development of a novel, high-performance personal aerosol sampler intended to monitor occupational air pollution, specifically, microbial constituents. This prototype sampler has a horizontally positioned conical cyclone with recirculating liquid film and an ejection supply of adsorptive liquid into the inlet nozzle. Airborne pollutants were collected in the adsorptive liquid, thus improving the survivability of microbiological aerosol samples.

[Influence of mutation L,D-carboxypeptidase-coding gene on dynamics of Listeria infection].

Aim: To study the progress of infection caused by mutant Listeria monocytogenes with deletion of Imo0028 gene coding L,D-carboxypeptidase (protein involved in metabolism of peptidoglycan).

Materials And Methods: Deletion of Imo0028 gene was performed with site-specific mutagenesis method using wild-type strain EGDe. Virulence was studied on mice of BALB/c line.

[Mismatch negativity in the auditory event-related potentials in response to abrupt and smooth displacements of virtual sound source].

The work investigated event-related potentials, mismatch negativity (MMN), and P3a component under dichotic stimulation with deviant stimuli simulating abrupt or smooth displacement of auditory images to the left or to the right from the head midline by means of interaural time delay introduced into the deviant stimuli. Repetitive standard stimuli were localized near the head midline. All deviant stimuli elicited mismatch negativity and P3a component.

[Auditory event-related potentials to the apparent auditory image motion].

Auditory event-related potentials (ERP) were registered to the dichotically presented white noise stimuli (duration 1500 ms, band 150-1200 Hz). Abrupt or gradual change ofinteraural time difference in the middle of stimuli (750 ms after sound offset) was perceived as an apparent auditory image (AI) instant relocation or motion from the midline to one of the ears. In responses these stimuli two ERPs were observed: one to the sound onset, and second--to the onset of motion or AI relocation.

[Perception of short musical fragments by hypomaniac patients].

Emotional assessment of short fragments from pieces of chamber music was studied in patients in steady elated mood (hypomaniac). Six fragments, assessed by healthy subjects as emotionally positive, neutral or negative were used. The examined subjects were shown to be capable for an emotional assessment of the musical fragments.

[Protective effect of the aerosol immunization of mice with the polycomponent vaccine Immunovac VP-4 after the challenge of the animals with Klebsiella pneumoniae virulent strain].

Used four schemes of the administration of the preparation with different time of the exposition of the animals in an aerosol chamber were tested with their subsequent intraperitoneal challenge with K. pneumoniae virulent strain K16. Irrespective of the number of immunization courses, the administration of the preparation made at intervals of 1 day, or daily, did not ensure any protective effect, but only led to an insignificant increase in their survival time in comparison with nonimmunized animals.

Switching time of nanowire memory.

Cadmium sulfide nanowires of 10-nm diameter, electrodeposited in porous anodic alumina films, exhibit an electronic bistability that can be harnessed for non-volatile memory. The current-voltage characteristics of the wires show two stable conductance states that are well-separated (conductances differ by more than 4 orders of magnitude) and long lived (longevity > 1 year at room temperature). These two states can encode binary bits 0 and 1.

Mismatch negativity evoked by stationary and moving auditory images of different azimuthal positions.

The present study has been designed to evaluate the pre-attentive detection of the location changes for stationary and moving sound sources. Auditory event-related potentials to the click trains simulating stationary and moving fused auditory images were recorded from healthy subjects using an oddball paradigm. The spatial characteristics of stimuli were created by introducing constant or variable interaural time delay (ITD) into the click trains.

High energy gain of trapped electrons in a tapered, diffraction-dominated inverse-free-electron laser.

Energy gain of trapped electrons in excess of 20 MeV has been demonstrated in an inverse-free-electron-laser (IFEL) accelerator experiment. A 14.5 MeV electron beam is copropagated with a 400 GW CO2 laser beam in a 50 cm long undulator strongly tapered in period and field amplitude.

Responses of cat primary auditory cortex neurons to moving stimuli with dynamically changing interaural delays.

The spike responses of individual neurons in the primary auditory cortex were studied in anesthetized cats during exposure to stationary and moving stimuli with static or dynamically changing interaural delays (deltaT). Static stimuli were tones and clicks. Dynamic stimuli were created using series of synphase and antiphase clicks with interaural delays which changed over time.

[Responses of neurons in the primary auditory cortex of the cat to the auditory motion stimuli with variable interaural delay].

Unit responses in the primary auditory cortex of anesthetized cats to stationary and apparently moving stimuli resulted from a static and dynamically varying interaural delay (ITD) were recorded. The static stimuli consisted of binaurally presented tones and clicks. The dynamic stimuli were produced by in-phase and out-of-phase binaurally presented click trains with time-varying ITD.

High-power electrostatic free-electron maser as a future source for fusion plasma heating: experiments in the short-pulse regime.

A high-power, frequency-tunable electrostatic free-electron maser, being developed at the FOM Institute for Plasma Physics "Rijnhuizen," shows lasing at various frequencies. An output power of 730 kW at 206 GHz is generated by a 7.2-A, 1.

[Brain activity induced by changes in acoustic stimuli].

The averaged variants of the EPs to deviant stimuli had more obvious components N100, N250 and P300 as compared with the EPs to standard stimuli. The data obtained show occurrence of a negative shift during presentation of a rare stimulus within the sequence of standard ones. This negativity seems to reflect the processes of comparison of acting excitations with the traces of previous stimuli.